NFIB Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19526

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (97%), Rat (100%), Xenopus (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NFIB Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFIB. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (95%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NFIB Antibody - BSA Free

Western Blot: NFIB Antibody [NBP2-19526]

Western Blot: NFIB Antibody [NBP2-19526]

Western Blot: NFIB Antibody [NBP2-19526] - Western Blot analysis of NFIB expression in transfected 293T cell line by NFIB polyclonal antibody. A: Non-transfected lysate. B: NFIB transfected lysate. 12% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: NFIB Antibody [NBP2-19526]

Immunohistochemistry-Paraffin: NFIB Antibody [NBP2-19526]

Immunohistochemistry-Paraffin: NFIB Antibody [NBP2-19526] - Paraffin-embedded mouse fore brain. NFIB antibody [N1C2] dilution: 1:500.
NFIB Antibody

Western Blot: NFIB Antibody [NBP2-19526] -

Western Blot: NFIB Antibody [NBP2-19526] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NFIB antibody [N1C2] (NBP2-19526) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NFIB Antibody - BSA Free

Immunohistochemistry: NFIB Antibody [NBP2-19526] -

NFIB antibody [N1C2] detects NFIB protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
NFIB antibody [N1C2] (NBP2-19526) dilution: 1:500.

Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15min

Applications for NFIB Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFIB

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Alternate Names

CCAAT-box-binding transcription factor, CTF, HMGIC/NFIB, NF-I/B, NFI-B, NFIB2, NFIB3, NFI-RED, nuclear factor 1 B-type, Nuclear factor 1/B, nuclear factor I/BNF1-B, TGGCA-binding protein

Gene Symbol

NFIB

UniProt

O00712 (Human)

Additional NFIB Products

Product Documents for NFIB Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NFIB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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