NFIX Antibody - BSA Free

Western Blot: NFIX Antibody - BSA Free [NBP2-15039] -

Nfix is expressed after phagocytosis and drive MP phenotypical switch. (a) Phagocytosis assay of M1 and M2c Nfixfl/fl and LysMCRE:Nfixfl/fl MPs cocultured 8h with apoptotic mpc. Representative FACS gate of phagocytotic M2c Nfixfl/fl and LysMCRE:Nfixfl/fl MPs (CD64+CellVue+) and percentage of phagocytotic M1 and M2c MPs coming from Nfixfl/fl and LysMCRE:Nfixfl/fl BMDM; (b) WT MPs were cocultured 16h with apoptotic mpcs. Representative FACS gate of non-phagocytotic (CD64+CellVue−) and phagocytotic (CD64+CellVue+) WT MPs. Quantification of Nfix expression realized by RT-qPCR on sorted non-phagocytotic and phagocytotic WT MPs and quantification of MPs positive for Nfix (Nfix+/F4/80+) realized by IF on non-phagocytotic and phagocytotic WT MPs; (c) WT MPs were cocultured for 16 h with apoptotic mpcs, with or without addition of Cytochalasin D. Quantification of F4/80+ MPs were positive for Nfix on a total of F4/80+ MPs; (d) Western blot of Nfix expression in WT MPs treated with DMSO (Dimethyl sulfoxide) or Y27632 for 16 h and quantification. Vinculin was used to normalize; (e) WT MPs were treated with DMSO or Y27632 for 16 h and were immunolabeled for pro-inflammatory markers (iNOS and TNF alpha ) and anti-inflammatory markers (TGF beta and CD163). The number of positive cells is expressed as percentage out of total cells; (f) LysMCRE:Nfixfl/fl MPs were treated with DMSO or Y27632 for 16 h and were immunolabeled for pro-inflammatory markers (iNOS and TNF alpha ) and anti-inflammatory markers (TGF beta and CD163). The number of positive cells is expressed as percentage out of total cells. * p < 0.05, *** p < 0.001. Results are means +/- SEM of at least three independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32183151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: NFIX Antibody - BSA Free [NBP2-15039] -

Nfix is mainly expressed by anti-inflammatory MPs. (a) Percentage of F4/80+ MPs positive for Nfix in Tibialis Anterior muscles (TA) of WT mice injected by CTX at D2, D4 and D7, post-injury. Immunostaining for F4/80 (green), Nfix (red) and DAPI (blue) at D4 and D7 after CTX injection; (b) Percentage of Ly6C+ and Ly6C- sorted MPs positive for Nfix in TA muscles of WT mice injected by CTX at D2, D4 and D7 post-injury; (c) Percentage of Nfix+ MPs after M1 and M2c polarization (with IFN gamma and IL10, respectively). * p < 0.05; *** p < 0.001; for (b) * p < 0.05 Ly6C+ vs. Ly6C+ at D4 and D7; # p < 0.05 Ly6C− D7 vs. D2. Results are means +/- SEM of at least three independent experiments. Scale bar = 50 μm. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32183151), licensed under a CC-BY license. Not internally tested by Novus Biologicals.