NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

Novus Biologicals | Catalog # NB300-114

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Canine (100%), Chicken (100%), Human (100%), Primate (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all NMDA R, NR1 Subunit Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit, N1 splice variant insert conjugated to KLH. Accession # P35439

Reactivity Notes

It is anticipated that the antibody will also work with canine, chicken, human, non-human primate and Xenopus tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Specificity

Specific for endogenous levels of the ~120 kDa NMDA R, NR1 Subunit splice variant insert protein of the NMDA receptor. Does not recognize NR1 subunits of the NMDA receptor that do not contain the N1 insert.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The antigen is a peptide from the NR1 subunit, N1 splice variant insert of rat NMDA receptor. Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).

Scientific Data Images for NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

Western Blot: NMDA R, NR1 Subunit AntibodyAzide and BSA Free [NB300-114]

Western Blot: NMDA R, NR1 Subunit AntibodyAzide and BSA Free [NB300-114]

Western Blot: NMDA receptor N1 Antibody [NB300-114] - 10 ug of HEK 293 cells expressing: Lane 1 - NR1 subunit containing the N1 and C2' Insert showing specific immunolabeling of the ~120k NR1 subunit of the NMDA receptor containing the N1 splice variant insert.; Lane 2 - NR1 subunit containing only the C2 Insert; Lane 3 - NR1 subunit containing the C1 and C2' Insert; Lane 4 - without NR1 expression (Mock).
Flow Cytometry: NMDA R, NR1 Subunit Antibody - Azide and BSA Free [NB300-114]

Flow Cytometry: NMDA R, NR1 Subunit Antibody - Azide and BSA Free [NB300-114]

Flow Cytometry: NMDA receptor N1 Antibody [NB300-114] - CD4+ Lymphocytes expressing NMDAR1. Image provided by verified customer.

Applications for NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:1000-1:2000

Western Blot

1:1000
Application Notes
NB 300-114 can be used in Western blot where a band is seen at ~ 100 kDa representing the NMDAR1 N1 splice variant.

Reviewed Applications

Read 1 review rated 4 using NB300-114 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute with 50 uL PBS to desired concentration.

Formulation

Lyophilized

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NMDA R, NR1 Subunit

The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning. NMDA receptors are also implicated in several disorders of the central nervous system including epilepsy and ischemic neuronal cell death. NMDA receptors also appear to be a target for ethanol at physiological concentrations and therefore may play a significant role in alcoholism.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 1

Alternate Names

GluN1, MRD8, NDHMSD, NDHMSR;NMD-R1, NMDA R, NR1 Subunit, NMDA1, NMDAR1, NMD-R1, NR1

Gene Symbol

GRIN1

UniProt

Q05586 (Human), P35439 (Rat)

Additional NMDA R, NR1 Subunit Products

Product Documents for NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free

Customer Reviews for NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free (1)

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Balb/c Mouse Lymphocytes
    Species: Mouse
    Verified Customer | Posted 01/31/2014
    CD4+ Lymphocytes expressing NMDAR1
    NMDA R, NR1 Subunit Antibody - Splice Variant - Azide and BSA Free NB300-114

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