![Immunohistochemistry-Paraffin: NPM-ALK Antibody (5A4) [NB120-17127]](https://resources.rndsystems.com/images/products/ALK-CD246-Antibody-5A4-Immunohistochemistry-Paraffin-NB120-17127-img0002.jpg "Immunohistochemistry-Paraffin: NPM-ALK Antibody (5A4) [NB120-17127]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5A4
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Product Specifications
Immunogen
Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of NPM-ALK transcript (419-520 aa).
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%)
Localization
Cell Membrane
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for NPM-ALK Antibody (5A4)
Immunohistochemistry-Paraffin: NPM-ALK Antibody (5A4) [NB120-17127]
Immunohistochemistry-Paraffin: ALK/CD246 Antibody (5A4) [NB120-17127] - Formalin fixed paraffin embedded anaplastic lymphoma kinase stained with ALK/CD246 antibody (NB120-17127).![Immunohistochemistry-Paraffin: NPM-ALK Antibody (5A4) [NB120-17127]](https://resources.rndsystems.com/images/products/ALK-CD246-Antibody-5A4-Immunohistochemistry-Paraffin-NB120-17127-img0001.jpg "Immunohistochemistry-Paraffin: NPM-ALK Antibody (5A4) [NB120-17127]")
Immunohistochemistry-Paraffin: NPM-ALK Antibody (5A4) [NB120-17127]
Immunohistochemistry-Paraffin: ALK/CD246 Antibody (5A4) [NB120-17127] - Formalin-fixed, paraffin-embedded human anaplastic lymphoma stained with p80/ALK using peroxidase-conjugate and AEC chromogen. Note nuclear and cytoplasmic staining of tumor cells.Applications for NPM-ALK Antibody (5A4)
Application
Recommended Usage
Immunohistochemistry
1:25-1:70
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.
Formulation, Preparation, and Storage
Purification
Tissue culture supernatant
Formulation
Tissue culture supernatant
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Product Documents for NPM-ALK Antibody (5A4)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NPM-ALK Antibody (5A4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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