p53 Antibody (S07-6A7)

Name: p53 Antibody (S07-6A7)
Brand: Novus Biologicals
SKU: NBP3-20045
Price: 569 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-20045

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S07-6A7 expressed in HEK293

View all p53 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human p53 (Uniprot # P04637)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for p53 Antibody (S07-6A7)

Western Blot: p53 Antibody (S07-6A7) [NBP3-20045]

Western Blot: p53 Antibody (S07-6A7) [NBP3-20045] - Western blot detection of p53 in Jurkat cell lysates using NBP3-20045 (1:500 diluted). Predicted band size: 44kDa. Observed band size: 53kDa.

Immunocytochemistry/Immunofluorescence: p53 Antibody (S07-6A7) [NBP3-20045] -

Immunocytochemistry/Immunofluorescence: p53 Antibody (S07-6A7) [NBP3-20045] - Immunofluorescence of p53 (green) in MCF-7 using NBP3-20045 at dilution 1/20, and DAPI (blue)

Applications for p53 Antibody (S07-6A7)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: p53

p53 is a stress-regulated transcription factor that was first identified as an SV40 large T antigen-binding protein. It plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. Mutation of p53 is the most common genetic change so far identified in a number of major carcinomas. This antibody can be used for the specific detection of human p53 that is synthesized in the presence of p53 from other species.

Alternate Names

BCC7, LFS1, TP53, TRP53

Gene Symbol

TP53

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Product Documents for p53 Antibody (S07-6A7)

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Certificate of Analysis

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Product Specific Notices for p53 Antibody (S07-6A7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.