PAPSS2 Antibody (OTI2E7)

Novus Biologicals | Catalog # NBP3-44693

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2E7
View all PAPSS2 Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-247 of human PAPSS2(NP_004661) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PAPSS2 Antibody (OTI2E7)

PAPSS2 Antibody (OTI2E7) Immunohistochemistry;PAPSS2 Antibody (OTI2E7)[NBP3-44693]-

Immunohistochemistry;PAPSS2 Antibody (OTI2E7)[NBP3-44693]-

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
PAPSS2 Antibody (OTI2E7) Western Blot;PAPSS2 Antibody (OTI2E7)[NBP3-44693]-

Western Blot;PAPSS2 Antibody (OTI2E7)[NBP3-44693]-

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAPSS2 (, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAPSS2.
PAPSS2 Antibody (OTI2E7) Immunohistochemistry;PAPSS2 Antibody (OTI2E7)[NBP3-44693]-

Immunohistochemistry;PAPSS2 Antibody (OTI2E7)[NBP3-44693]-

Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.
PAPSS2 Antibody (OTI2E7) Western Blot;PAPSS2 Antibody (OTI2E7)[NBP3-44693]-

Western Blot;PAPSS2 Antibody (OTI2E7)[NBP3-44693]-

Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, and PAPSS2-Knockout HeLa cells (KO, ) were separated by SDS-PAGE and immunoblotted with anti-PAPSS2 monoclonal antibody TA807130 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
PAPSS2 Antibody (OTI2E7) Immunohistochemistry;PAPSS2 Antibody (OTI2E7)[NBP3-44693]-

Immunohistochemistry;PAPSS2 Antibody (OTI2E7)[NBP3-44693]-

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PAPSS2 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Applications for PAPSS2 Antibody (OTI2E7)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PAPSS2

Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene.

Alternate Names

3'-phosphoadenosine 5'-phosphosulfate synthase 2, ATP sulfurylase/adenosine 5'-phosphosulfate kinase, ATPSK2ATP sulfurylase/APS kinase 2, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthethase 2, EC 2.7.1.25, PAPS synthase 2, PAPS synthetase 2, PAPSS 2, SK 2, SK2, Sulfurylase kinase 2,3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 2

Gene Symbol

PAPSS2

Additional PAPSS2 Products

Product Documents for PAPSS2 Antibody (OTI2E7)

Certificate of Analysis

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Product Specific Notices for PAPSS2 Antibody (OTI2E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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