PCBP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80455

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PCBP1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide directed towards the middle region of human PCBP1. Peptide sequence PHATHDLEGPPLDAYSIQGQHTISPLDLAKLNQVARQQSHFAMMHGGTGF. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for PCBP1 Antibody - BSA Free

Western Blot: PCBP1 Antibody [NBP1-80455]

Western Blot: PCBP1 Antibody [NBP1-80455]

Western Blot: PCBP1 Antibody [NBP1-80455] - Host: Mouse. Target Name: PCBP1. Sample Tissue: Mouse Testis. Antibody Dilution: 1ug/ml
Immunohistochemistry: PCBP1 Antibody [NBP1-80455]

Immunohistochemistry: PCBP1 Antibody [NBP1-80455]

Immunohistochemistry: PCBP1 Antibody [NBP1-80455] - Anti-PCBP1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western Blot: PCBP1 Antibody [NBP1-80455]

Western Blot: PCBP1 Antibody [NBP1-80455]

Western Blot: PCBP1 Antibody [NBP1-80455] - HepG2 Cell Lysate 1ug/ml Gel Concentration 12%
Western Blot: PCBP1 Antibody [NBP1-80455]

Western Blot: PCBP1 Antibody [NBP1-80455]

Western Blot: PCBP1 Antibody [NBP1-80455] - MCF7 Whole Cell lysates, Antibody Dilution: 1.0 ug/ml PCBP1 is supported by BioGPS gene expression data to be expressed in MCF7.
Western Blot: PCBP1 Antibody [NBP1-80455]

Western Blot: PCBP1 Antibody [NBP1-80455]

Western Blot: PCBP1 Antibody [NBP1-80455] - Host: Rabbit. Target Name: PCBP1. Sample Tissue: Human HCT116. Antibody Dilution: 1.0ug/ml
Western Blot: PCBP1 Antibody [NBP1-80455]

Western Blot: PCBP1 Antibody [NBP1-80455]

Western Blot: PCBP1 Antibody [NBP1-80455] - Host: Rabbit. Target Name: PCBP1. Sample Tissue: Human THP-1 Whole Cell. Antibody Dilution: 1ug/ml
Immunohistochemistry-Paraffin: PCBP1 Antibody [NBP1-80455]

Immunohistochemistry-Paraffin: PCBP1 Antibody [NBP1-80455]

Immunohistochemistry-Paraffin: PCBP1 Antibody [NBP1-80455] - Human Breast.
Immunohistochemistry-Paraffin: PCBP1 Antibody [NBP1-80455]

Immunohistochemistry-Paraffin: PCBP1 Antibody [NBP1-80455]

Immunohistochemistry-Paraffin: PCBP1 Antibody [NBP1-80455] - Staining of human spleen.
Immunohistochemistry-Paraffin: PCBP1 Antibody [NBP1-80455]

Immunohistochemistry-Paraffin: PCBP1 Antibody [NBP1-80455]

Immunohistochemistry-Paraffin: PCBP1 Antibody [NBP1-80455] - Human Breast tissue at an antibody concentration of 5.0ug/ml.

Applications for PCBP1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PCBP1

PCBP1 is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq]

Alternate Names

Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein E1, heterogenous nuclear ribonucleoprotein X, hnRNP E1, hnRNP-E1, hnRNP-X, HNRPE1, HNRPX, nucleic acid binding protein sub 2.3, Nucleic acid-binding protein SUB2.3, poly(rC) binding protein 1, poly(rC)-binding protein 1

Gene Symbol

PCBP1

UniProt

NP_006187 (Human)

Additional PCBP1 Products

Product Documents for PCBP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PCBP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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