PCNA associated factor Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32112

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PCNA associated factor Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 61 to 111 of human PCNA-Associated Factor using the numbering given in entry NP_055551.1 (GeneID 9768).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PCNA associated factor Antibody - BSA Free

Western Blot: PCNA associated factor Antibody [NBP2-32112]

Western Blot: PCNA associated factor Antibody [NBP2-32112]

Western Blot: PCNA associated factor Antibody [NBP2-32112] - Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PAF antibody NBP2-32112 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP2-32112]

Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP2-32112]

Immunohistochemistry-Paraffin: PCNA associated factor Antibody [NBP2-32112] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- PAF used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Applications for PCNA associated factor Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Western Blot

1:2000 - 1:10000
Application Notes
Immunoprecipitation Not recommended. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PCNA associated factor

KIAA0101/NS5ATP9/PAF/p15(PAF) is involved in protection of cells from UV-induced cell death and is also a Hepatitis C NS5A up-regulation gene which may play a role in the pathogenesis of HCV-associated hepatocellular carcinoma.

Alternate Names

HCV NS5A-transactivated protein 9, Hepatitis C virus NS5A-transactivated protein 9, KIAA0101, NS5ATP9p15PAF, OEATC-1OEATC1, Overexpressed in anaplastic thyroid carcinoma 1, p15(PAF), PAF, PCNA-associated factor

Gene Symbol

PCLAF

UniProt

NP_055551.1 (Human)

Additional PCNA associated factor Products

Product Documents for PCNA associated factor Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PCNA associated factor Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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