PCP4 Antibody (CL5306)

Novus Biologicals | Catalog # NBP3-52479

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 Clone # CL5306
View all PCP4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P48539, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PCP4 Antibody (CL5306)

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]

Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]

Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]

Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]

Staining of human tonsil shows no positivity in non-germinal center cells as expected.
Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]

Staining of mouse brain shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]

Immunohistochemistry-Paraffin: PCP4 Antibody (CL5306) [NBP3-52479]

Staining of rat brain shows strong cytoplasmic positivity in Purkinje cells.

Applications for PCP4 Antibody (CL5306)

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PCP4

Alternate Names

brain specific polypeptide PEP19, 10Brain-specific antigen PCP-4, Brain-specific polypeptide PEP-19, PEP19, PEP-19, Purkinje cell protein 4

Gene Symbol

PCP4

Additional PCP4 Products

Product Documents for PCP4 Antibody (CL5306)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PCP4 Antibody (CL5306)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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