Key Product Details
Applications
Product Specifications
Specificity
Application Notes
1. Solution Pepsin is ready to use and does not require any preparation.
Protocol Recommendations
1. Deparaffinize tissue sections and wash slides with wash buffer.
2. Remove excess buffer from slide(s) without letting the tissue dry.
3. Depending on the size of the tissue section add 1-2 drops of pepsin solution.
4. Incubate slide(s) for 5-10 minutes at 37C.
5. Wash slide(s) with wash buffer and proceed with immunostaining.
Formulation, Preparation, and Storage
Preservative
Concentration
Shipping
Storage
Background: Pepsin Solution
Additional Pepsin Solution Products
Product Documents for Pepsin Solution
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Pepsin Solution
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Pepsin Solution
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Q: Could you please let me know the concentration of NB900-66724?
A: Our pepsin solution is provided as 3.0mg/ml.
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Q: Do you have pepsin 5% solution?
A: NB900-66724 is a pepsin solution used for unmasking epitopes for IHC on paraffin-embedded tissues. I do not currently have information on its concentration.
-
Q: Could you please let me know the concentration of NB900-66724?
A: Our pepsin solution is provided as 3.0mg/ml.
-
Q: Do you have pepsin 5% solution?
A: NB900-66724 is a pepsin solution used for unmasking epitopes for IHC on paraffin-embedded tissues. I do not currently have information on its concentration.