PHF10 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19795

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PHF10 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHF10. The exact sequence is proprietary.

Reactivity Notes

Bovine (80%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PHF10 Antibody - BSA Free

Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795]

Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795]

Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795] - Mouse lung. PHF10 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: PHF10 Antibody [NBP2-19795]

Western Blot: PHF10 Antibody [NBP2-19795]

Western Blot: PHF10 Antibody [NBP2-19795] - 50 ug mouse colon lysate/extract 10% SDS-PAGE PHF10 antibody dilution: 1:1000.
Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795]

Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795]

Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795] - Hela xenograft, using PHF10 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795]

Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795]

Immunohistochemistry-Paraffin: PHF10 Antibody [NBP2-19795] - Rat lung. PHF10 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
PHF10 Antibody

Western Blot: PHF10 Antibody [NBP2-19795] -

Western Blot: PHF10 Antibody [NBP2-19795] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PHF10 antibody (NBP2-19795) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
PHF10 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PHF10 Antibody [NBP2-19795] -

PHF10 antibody detects PHF10 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PHF10 protein stained by PHF10 antibody (NBP2-19795) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
PHF10 Antibody - BSA Free

Western Blot: PHF10 Antibody - BSA Free [NBP2-19795] -

Baf45a expression levels induce gene expression profiles for osteoblast differentiation. (A) Relative expression levels of Baf45a (left panel) and Runx2 (right panel) were obtained by real-time RT-qPCR using DNase I treated total RNA isolated from murine preosteoblast MC3T3-E1 cells induced to differentiate for 18 days. Gapdh mRNA profile was used as an experimental control. (B) Representative Western blot analysis with anti-BAF45A antibody of lysates from the femur and MC3T3-E1 cells. Molecular weight markers were indicated in kDa. (C) Relative protein levels of BAF45A (upper panel) and RUNX2 (middle panel) were obtained by Western blot analysis using total cell lysate isolated from murine calvarial osteoblast cells induced to differentiate for 25 days. Beta Tubulin protein profile (lower panel) was used as loading control. (D) Relative expression levels of Runx2 were obtained by real-time RT-qPCR using DNase I treated total RNA isolated from murine calvarial cells induced to differentiate for 25 days. (E) Overexpression of Baf45a in MC3T3-E1 osteoblast cells normalized to Gapdh after 72 hours by RT-qPCR. (F–J) Expressions of Runx2, Sp7 (osterix) osterix, Hoxa5, Hoxa10, and Hoxa11 were assessed by RT-qPCR and normalized to Gapdh. (J) Early markers Alp and Col1A1 and (K) late markers Spp1(Opn), and Ocn were assayed. Statistical significance was determined by Student’s t-test (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 versus matched control). Gapdh expression was used as the control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35046892), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PHF10 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 4 using NBP2-19795 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PHF10

This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. [provided by RefSeq]

Alternate Names

BAF45a, BAF45APHD zinc finger protein XAP135, BRG1-associated factor 45a, FLJ10975, MGC111009, PHD finger protein 10, XAP135BRG1-associated factor, 45-KD, A

Gene Symbol

PHF10

UniProt

Q8WUB8 (Human)

Additional PHF10 Products

Product Documents for PHF10 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PHF10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PHF10 Antibody - BSA Free

Customer Reviews for PHF10 Antibody - BSA Free (1)

4 out of 5
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  • Name: Xiaofeng Wang
    Application: Western Blot
    Sample Tested: BT16 Nuclear Extract
    Species: Human
    Verified Customer | Posted 08/07/2014
    PHF10 WB
    PHF10 Antibody - BSA Free NBP2-19795

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