Pichia Pastoris Antibody - BSA Free

Novus Biologicals | Catalog # NB100-65067

Novus Biologicals
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Key Product Details

Species Reactivity

Yeast

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Pichia Pastoris Primary Antibodies »

Product Specifications

Immunogen

P. pastoris whole cells

Specificity

Reacts with all Pichia pastoris antigens and displays cross reactivity with C. albicans and to a lesser extent S. cerevisiae. This is useful for detection/removal of contaminants from recombinant preparations.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Pichia Pastoris Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

4.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Pichia Pastoris

Pichia pastoris, a methylotrophic yeast, has been used as a heterologous protein expression system. It combines many of the benefits of E. coli expression with the advantages of expression in an eukaryotic system such as protein processing, folding, and posttranslational modifications. Pichia pastoris is capable of metabolizing methanol as the sole carbon source. The first step in the metabolism of methanol is the oxidation of methanol to formaldehyde by the enzyme, alcohol oxidase. Expression of this enzyme, coded for by the AOX1 gene, is tightly regulated and induced by methanol to very high levels: typically >30% of the total soluble protein in the cells.

Alternate Names

Komagataella phaffii

Additional Pichia Pastoris Products

Product Documents for Pichia Pastoris Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Pichia Pastoris Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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