PIDD Antibody (AL233) - BSA Free

N/A: LRDD Antibody (AL233) [NBP3-11679] - Inhibition of binding of recombinant human IL-33 (hIL-33) to human ST2 (hST2) in vitro using LRDD antibody (AL233). An indirect competitive ELISA was performed as follows; 1) coat microtiter plate wells with hST2-Fc (10 ug/ml); 2) add 2 ug/ml of IL-33 with or without a hIL-33 mAb to the wells followed by washing; 3) add anti-FLAG HRP conjugated (1:2,000) to an enzyme; 4) after adding the TMB solution, incubate at RT in the dark for 20 minutes. Immediately read the plate at 450 nm.