PIM1 Antibody - BSA Free

PIM1 Antibody:
Immunocytochemistry Protocol

Culture cells to appropriate density on suitable glass coverslips in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 5-10 minutes.
2. Remove the formalin and add 0.5% Triton-X 100 in TBS to permeabilize the cells. Incubate for 5-10 minutes.
3. Remove the permeabilization buffer and add wash buffer (i.e. PBS or PBS with 0.1% Tween-20). Be sure to not let the specimen dry out. Gently wash three times for 10 minutes.
4. Alternatively, cells can be fixed with -20C methanol for 10 min at room temperature. Remove the methanol and rehydrate in PBS for 10 min before proceeding.
5. To block nonspecific antibody binding incubate in 10% normal goat serum for 1 hour at room temperature.
6. Add primary antibody at appropriate dilution and incubate at room temperature for 1 hour or at 4 degrees C overnight.
7. Remove primary antibody and replace with wash buffer. Gently wash three times for 10 minutes.
8. Add secondary antibody at the appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove antibody and replace with wash buffer. Gently wash three times for 10 minutes.
10. Nuclei can be staining with 4',6' diamino phenylindole (DAPI) at 0.1 ug/ml, or coverslips can be directly mounted in media containing DAPI.
11. Cells can now be viewed with a fluorescence microscope.

*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow proper laboratory procedures for the disposal of formalin.

PIM1 Antibody:
Western Blot Protocol
1. Perform SDS-PAGE on protein samples to be analyzed, loading 10-40 ug of total protein per lane.
2. Electro-blot the proteins to a suitable membrane (PVDF or Nitrocellulose) according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or a similar product) to assess transfer success. Mark molecular weight standards where appropriate.
4. Thoroughly rinse the membrane of stain with TBST.
5. Incubate the membrane in blocking buffer (5% non-fat milk in TBST or 5% BSA in TBST) as appropriate, for 60 minutes.
6. Dilute the primary antibody as appropriate in blocking buffer and incubate for 60 minute at room temperature to overnight at 4 degrees C with gently shaking.
7. Wash the membrane in TBST three times for 10 minutes each.
8. Incubate the membrane in the appropriate secondary antibody prepared in blocking buffer (as per manufacturer's instructions) and incubate for 60 minutes at room temperature.
9. Wash the membrane in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
10. Incubate the membrane in the appropriate detection reagent in accordance with the manufacturer's instructions and image the blot.
Note: Tween-20 can be added to the blocking, wash and antibody dilution buffers to a final concentration of 0.05-0.1%.