PKA 2 beta Antibody (2U2P1)

Novus Biologicals | Catalog # NBP3-15890

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2U2P1 expressed in HEK293
View all PKA 2 beta Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 319-418 of human PKA 2 beta (P31323). GEVKITMKRKGKSEVEENGAVEIARCSRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PKA 2 beta Antibody (2U2P1)

Western Blot: PKA 2 beta Antibody (2U2P1) [NBP3-15890]

Western Blot: PKA 2 beta Antibody (2U2P1) [NBP3-15890]

Western Blot: PKA 2 beta Antibody (2U2P1) [NBP3-15890] - Western blot analysis of extracts of various cell lines, using PKA 2 beta Rabbit mAb (NBP3-15890) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: PKA 2 beta Antibody (2U2P1) [NBP3-15890]

Immunohistochemistry-Paraffin: PKA 2 beta Antibody (2U2P1) [NBP3-15890]

Immunohistochemistry-Paraffin: PKA 2 beta Antibody (2U2P1) [NBP3-15890] - Immunohistochemistry of paraffin-embedded mouse brain using PKA 2 beta Rabbit mAb (NBP3-15890) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: PKA 2 beta Antibody (2U2P1) [NBP3-15890]

Immunohistochemistry-Paraffin: PKA 2 beta Antibody (2U2P1) [NBP3-15890]

Immunohistochemistry-Paraffin: PKA 2 beta Antibody (2U2P1) [NBP3-15890] - Immunohistochemistry of paraffin-embedded rat ovary using PKA 2 beta Rabbit mAb (NBP3-15890) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for PKA 2 beta Antibody (2U2P1)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PKA 2 beta

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol.

Long Name

cAMP-dependent protein kinase type II-beta regulatory subunit

Alternate Names

PRKAR2B

Gene Symbol

PRKAR2B

Additional PKA 2 beta Products

Product Documents for PKA 2 beta Antibody (2U2P1)

Certificate of Analysis

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Product Specific Notices for PKA 2 beta Antibody (2U2P1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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