PLA2R1 Antibody (CL0474) - BSA Free

Novus Biologicals | Catalog # NBP2-52933

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0474

Format

BSA Free
View all PLA2R1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q13018, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PLA2R1 Antibody (CL0474) - BSA Free

PLA2R1 Antibody (CL0474) - BSA Free Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Staining of HeLa cells using the Anti-PLA2R1 monoclonal antibody, showing specific staining in the cytosol and plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
PLA2R1 Antibody (CL0474) - BSA Free Immunohistochemistry: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Immunohistochemistry: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Staining of human kidney (idiopathic membranous nephropathy) shows strong membranous positivity in cells in glomeruli.
PLA2R1 Antibody (CL0474) - BSA Free Immunohistochemistry: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Immunohistochemistry: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Staining of human kidney shows weak membranous positivity in cells in glomeruli.
PLA2R1 Antibody (CL0474) - BSA Free Immunohistochemistry: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Immunohistochemistry: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
PLA2R1 Antibody (CL0474) - BSA Free Immunohistochemistry: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Immunohistochemistry: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Analysis in human kidney and pancreas tissues using AMAb90772 antibody. Corresponding PLA2R1 RNA-seq data are presented for the same tissues.
PLA2R1 Antibody (CL0474) - BSA Free Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Immunocytochemistry/ Immunofluorescence: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Validation of the Anti-PLA2R1 monoclonal antibody by comparing the Staining in human cell lines Hela and MCF7 exhibiting a relatively high and low expression of PLA2R1 (based on RNA-seq values), respectively. The PLA2R1 (green signal) is present in Hela cells but is absent in MCF7 cells. The anti-HDAC1 antibody (NBP2-52933) was used as a loading control (red signal).
PLA2R1 Antibody (CL0474) - BSA Free Western Blot: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Western Blot: PLA2R1 Antibody (CL0474) - BSA Free [NBP2-52933]

Analysis in human kidney tissue.

Applications for PLA2R1 Antibody (CL0474) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:20000 - 1:50000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PLA2R1

Secretory phospholipases A2 (PLA2s) have been purified from a variety of mammalian tissues as well as from insect and snake venoms. The prototype group I PLA2, the pancreatic PLA2 (PLA2G1B; MIM 172410), is involved in digestion, smooth muscle contraction, and cell proliferation. The prototype group II PLA2, the inflammatory-type PLA2 (PLA2G2A; MIM 172411), is involved in inflammatory conditions and is upregulated by proinflammatory cytokines like tumor necrosis factor (TNF; MIM 191160) and interleukin-1 (e.g., IL1B; MIM 147720). Differential toxicity of snake venom Pla2 appears to be linked to a variety of high-affinity receptors in different organs (Ancian et al., 1995 (PubMed 7721806)).(supplied by OMIM)

Long Name

Phospholipase A2 Receptor 1, 180kDa

Alternate Names

CLEC13C, PLA2G1R

Gene Symbol

PLA2R1

Additional PLA2R1 Products

Product Documents for PLA2R1 Antibody (CL0474) - BSA Free

Certificate of Analysis

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Product Specific Notices for PLA2R1 Antibody (CL0474) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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