Plexin D1 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-33634
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Predicted:
Rat (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Plexin D1. The exact sequence is proprietary.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 31163177).
Localization
Cell membrane, Single-pass membrane protein
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
212 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit Plexin D1 Antibody - BSA Free (NBP1-33634) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Plexin D1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Plexin D1 Antibody - BSA Free
Western Blot: Plexin D1 Antibody [NBP1-33634]
Western Blot: Plexin D1 Antibody [NBP1-33634] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Plexin D1 antibody [C3], C-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Plexin D1 Antibody [NBP1-33634]
Immunocytochemistry/Immunofluorescence: Plexin D1 Antibody [NBP1-33634] - Analysis of methanol-fixed HeLa, using Plexin D1antibody at 1:500 dilution.Immunohistochemistry-Frozen: Plexin D1 Antibody [NBP1-33634]
Immunohistochemistry-Frozen: Plexin D1 Antibody [NBP1-33634] - PlexinD1 on an E12.5 mouse Left Ventricle. Antibody at 1:100. Alexa Fluor 488 secondary antibody at 1:1000. IHC-Fr image submitted by a verified customer review.Applications for Plexin D1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry-Frozen
Validated from a verified customer review
Western Blot
1:500-1:3000
Reviewed Applications
Read 1 review rated 5 using NBP1-33634 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Plexin D1
Additional Plexin D1 Products
Product Documents for Plexin D1 Antibody - BSA Free
Product Specific Notices for Plexin D1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for Plexin D1 Antibody - BSA Free
Customer Reviews for Plexin D1 Antibody - BSA Free (1)
5 out of 5
1 Customer Rating
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Application: Immunohistochemistry-FrozenSample Tested: E12.5 mouse embryo fixed in 4% PFASpecies: MouseVerified Customer | Posted 06/23/2021PlexinD1 on an E12.5 mouse Left Ventricle.Fixed 4% PFA overnight. Blocked with 1% BSA Primary antibody dilution - 1:100 Secondary antibody - Invitrogen Alexa Fluor 488 Secondary antibody dilution - 1:1000 Stained on a E12.5 mouse left ventricleBio-Techne ResponseThis review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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