Podocalyxin Like Antibody (MSVA-645M)
Novus Biologicals | Catalog # NBP3-57266
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # MSVA-645M
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Product Specifications
Immunogen
Recombinant human PODXL fragment Swissprot O00592
Localization
Membranous
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
This antibody is highly specific and optimized for use in Immunohistochemistry.
It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.
The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.
Positive Control
Postive control:Kidney: A strong PODXL must be seen in glomerular podocytes while at least a weak to moderate PODXL staining must be seen in endothelial cells of small intertubular vessels..
Negative Control
Negitve control:Kidney: Tubuli and collecting ducts must be PODXL negative.
Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.
Scientific Data Images for Podocalyxin Like Antibody (MSVA-645M)
Immunohistochemistry-Paraffin:Podocalyxin Like Antibody (MSVA-645M)NBP3-57266
Renal cortex displaying an intense PODXL staining of glomerular endothelial cells while endothelial cells of other small vessels stain much less prominently.
Immunohistochemistry-Paraffin:Podocalyxin Like Antibody (MSVA-645M)NBP3-57266
Proliferating endometrium displaying a strong PODXL staining of apical membranes of endometrium glands.
Immunohistochemistry-Paraffin:Podocalyxin Like Antibody (MSVA-645M)NBP3-57266
Malignant mesothelioma with moderate to strong membranous PODXL staining ofabout 40 of the tumor cells.
Podocalyxin Like Cancer Tissue Gallery
Podocalyxin Like Cancer Tissue Gallery
Podocalyxin Like Cancer Tissue Gallery
Podocalyxin Like Normal Tissue Gallery
Podocalyxin Like Normal Tissue Gallery
Podocalyxin Like Normal Tissue Gallery
Podocalyxin Like Normal Tissue Gallery
Podocalyxin Like Normal Tissue Gallery
Podocalyxin Like Normal Tissue Gallery
Applications for Podocalyxin Like Antibody (MSVA-645M)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Preservative
<0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Podocalyxin
Alternate Names
GCTM, PCLP, POD XL, PODXL
Gene Symbol
PODXL
Additional Podocalyxin Products
Product Documents for Podocalyxin Like Antibody (MSVA-645M)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Podocalyxin Like Antibody (MSVA-645M)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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