POM121 Antibody

Western Blot: POM121 Antibody [NBP2-19890] -

Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with POM121 antibody [N2N3] (NBP2-19890) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: POM121 Antibody [NBP2-19890] -

SCA7 patient NPCs display a marked reduction in the expression level of nucleoporin POM121. (a) We performed immunoblot analysis on neuron progenitor cells (NPCs) derived from either an unaffected related control (10Q/10Q) and on NPCs derived from a SCA7 patient (65Q/10Q) after obtaining protein lysates, and preparing and loading samples with identical amounts of total protein. Levels of full-length POM121 protein, which migrates at ~145 kDa, are visibly reduced in four SCA7 NPC biological replicates compared to four NPC biological replicates from the unaffected related control. (b) To assure equivalent loading and protein level normalization, we used a 4–20% Stain-Free TGX gel (shown here). (c) After quantifying POM121 protein levels by densitometry and normalizing to total protein using stain-free technology (BioRad), we noted a significant >40% reduction in POM121 protein expression in SCA7 NPCs. Data was normalized to unaffected control mean, which was arbitrarily set to 1.0, and then compared by nested t-test, p = 0.0286. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39649105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.