PPRC1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82985

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PPRC1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ASPHPKHKVSALVQSPQMKALACVSAEGVTVEEPASERLKPETQETRPREKPPLPATKAVPTPRQSTVPKLPAVHPARLRKLSFLPTPRTQGSEDVVQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PPRC1 Antibody - BSA Free

Western Blot: PPRC1 Antibody [NBP1-82985]

Western Blot: PPRC1 Antibody [NBP1-82985]

Western Blot: PPRC1 Antibody [NBP1-82985] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: PPRC1 Antibody [NBP1-82985]

Immunocytochemistry/ Immunofluorescence: PPRC1 Antibody [NBP1-82985]

Immunocytochemistry/Immunofluorescence: PPRC1 Antibody [NBP1-82985] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985]

Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985]

Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985] - Staining of human liver shows no positivity in hepatoyctes as expected.
Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985]

Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985]

Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985] - Staining of human placenta shows strong cytoplasmic and nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985]

Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985]

Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985] - Staining of human lymph node shows strong nuclear positivity in germinal center cells and non-germinal center cells.
Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985]

Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985]

Immunohistochemistry-Paraffin: PPRC1 Antibody [NBP1-82985] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Applications for PPRC1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPRC1

The protein encoded by the PPRC1 gene is similar to PPAR-gamma coactivator 1 (PPARGC1/PGC-1), a protein that can activate mitochondrial biogenesis in part through a direct interaction with nuclear respiratory factor 1 (NRF1). This protein has been shown to interact with NRF1. It is thought to be a functional relative of PPARGC1 that activates mitochondrial biogenesis through NRF1 in response to proliferative signals. (provided by RefSeq)

Alternate Names

KIAA0595gamma, coactivator-related 1, peroxisome proliferator-activated receptor gamma, coactivator-related 1, PGC-1 related co-activator, PGC-1-related coactivator

Gene Symbol

PPRC1

Additional PPRC1 Products

Product Documents for PPRC1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PPRC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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