Propidium Iodide

Novus Biologicals | Catalog # NBP2-31155

Novus Biologicals
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Key Product Details

Species

Human

Applications

Flow Cytometry, Microscopy

Product Summary for Propidium Iodide

Propidium Iodide is excluded by viable cells but can penetrate cell membranes of dying or dead cells. Dead cells will take up PI and fluoresce brightly in FL2 off of the blue laser on a standard flow cytometer. PI also may be excited by the UV or Green/Yellow lasers on more advanced flow cytometers and may, therefore, not be optimal for certain multicolor panels. It emission maxima is at 617nm.

Product Specifications

Application Notes

Fluorescent stain for nucleic acids. Cell membrane integrity excludes propidium iodide from staining viable and apoptotic cells. Propidium iodide may be used in flow cytometry to evaluate cell viability when used with other dyes that stain viable cells or cells that are early in the apoptosis process.

Reactivity Notes

Use in Human reported in scientific literature (PMID:33800462).

Scientific Data Images for Propidium Iodide

Flow Cytometry: Propidium Iodide [NBP2-31155]

Flow Cytometry: Propidium Iodide [NBP2-31155]

Flow Cytometry: Propidium Iodide [NBP2-31155] - Image from verified customer review.
Propidium iodide Solution [NBP2-31155] - Propidium iodide Solution
Cellular Imaging: Propidium Iodide [NBP2-31155] - MDA-MB468 cells stained with Live green dye (Calcein) and dead PI dyes (red) after 5 days of drug treatment. Image from verified customer review.

Formulation, Preparation, and Storage

Preservative

No Preservative

Concentration

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4C in the dark.

Product Documents for Propidium Iodide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Propidium Iodide

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

Citations for Propidium Iodide

Customer Reviews for Propidium Iodide (2)

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2 Customer Ratings
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  • Propidium Iodide
    Name: Anonymous
    Verified Customer | Posted 10/09/2018
    Cells stained with Live green dye (Calcein) and dead PI dyes (red) after 5 days of drug treatment
    Propidium Iodide NBP2-31155
  • Propidium Iodide
    Name: Anonymous
    Verified Customer | Posted 06/29/2018
    Propidium Iodide NBP2-31155

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