PTK9 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90297

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PTK9 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: AEEELRQIKINEVQTDVGVDTKHQTLQGVAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PTK9 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PTK9 Antibody [NBP1-90297]

Immunocytochemistry/ Immunofluorescence: PTK9 Antibody [NBP1-90297]

Immunocytochemistry/Immunofluorescence: PTK9 Antibody [NBP1-90297] - Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]

Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]

Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]

Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]

Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]

Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]

Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]

Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297]

Immunohistochemistry-Paraffin: PTK9 Antibody [NBP1-90297] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Simple Western: PTK9 Antibody [NBP1-90297]

Simple Western: PTK9 Antibody [NBP1-90297]

Simple Western: PTK9 Antibody [NBP1-90297] - Electropherogram image(s) of corresponding Simple Western lane view. PTK9 antibody was used at 1:40 dilution on RT-4 and U-251MG lysate(s).
PTK9 Antibody - BSA Free Western Blot: PTK9 Antibody - BSA Free [NBP1-90297]

Western Blot: PTK9 Antibody - BSA Free [NBP1-90297]

Analysis in human cell line A-431.
PTK9 Antibody - BSA Free Western Blot: PTK9 Antibody - BSA Free [NBP1-90297]

Western Blot: PTK9 Antibody - BSA Free [NBP1-90297]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for PTK9 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Simple Western

1:40

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:40, apparent MW was 45 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTK9

PTK9 encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.

Alternate Names

A6, A6 protein tyrosine kinase, Protein A6, Protein tyrosine kinase 9MGC23788, PTK9 protein tyrosine kinase 9, PTK9MGC41876, twinfilin, actin-binding protein, homolog 1 (Drosophila), twinfilin-1

Gene Symbol

TWF1

Additional PTK9 Products

Product Documents for PTK9 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PTK9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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