PTPRH Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20005

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PTPRH. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)

Localization

Membrane; Single-pass type I membrane protein (Potential); Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PTPRH Antibody - BSA Free

Western Blot: PTPRH Antibody [NBP2-20005]

Western Blot: PTPRH Antibody [NBP2-20005]

Western Blot: PTPRH Antibody [NBP2-20005] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: PTPRH Antibody [NBP2-20005]

Immunocytochemistry/ Immunofluorescence: PTPRH Antibody [NBP2-20005]

Immunocytochemistry/Immunofluorescence: PTPRH Antibody [NBP2-20005] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
PTPRH Antibody - BSA Free

Immunohistochemistry-Paraffin: PTPRH Antibody [NBP2-20005] -

PTPRH antibody [C2C3], C-term detects PTPRH protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
PTPRH antibody [C2C3], C-term (NBP2-20005) diluted at 1:500.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
PTPRH Antibody - BSA Free

Western Blot: PTPRH Antibody - BSA Free [NBP2-20005] -

PTPRH promoted proliferation in NSCLC. A, B Detection of PTPRH expression using RT‒PCR and western blotting. C, D Expression of PTPRH after transfection by western blotting analysis and RT‒PCR. E The results of the colony formation assay.) F 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. G, H 5-ethynyl-2′-deoxyuridine (EdU) assay show that PTPRH enhances the proliferation ability of NSCLC cells Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37974250), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PTPRH Antibody - BSA Free

Knockdown Validated: PTPRH Antibody - BSA Free [NBP2-20005] -

PTPRH promoted proliferation in NSCLC. A, B Detection of PTPRH expression using RT‒PCR and western blotting. C, D Expression of PTPRH after transfection by western blotting analysis and RT‒PCR. E The results of the colony formation assay.) F 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. G, H 5-ethynyl-2′-deoxyuridine (EdU) assay show that PTPRH enhances the proliferation ability of NSCLC cells Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37974250), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PTPRH Antibody - BSA Free

Knockdown Validated: PTPRH Antibody - BSA Free [NBP2-20005] -

PTPRH inhibited cell cycle arrest and apoptosis. A, B Gene Set Enrichment Analysis (GSEA) of PTPRH. C, D Flow cytometry analysis of the apoptotic effect of PTPRH on NSCLC and statistical analysis. E, F Flow cytometry analysis of the effects of PTPRH expression on the cell cycle in NSCLC and statistical analysis. G Western blotting results showing the effects of short hairpin RNA-PTPRH (sh-PTPRH) treatment on cell cycle-related proteins. H Western blotting results showing the effects of sh-PTPRH treatment on the expression of apoptotic proteins Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37974250), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
PTPRH Antibody - BSA Free

Flow Cytometry: PTPRH Antibody - BSA Free [NBP2-20005] -

Correlation of PTPRH expression with 18F-fluorodeoxyglucose (18F-FDG) semiquantitative indicators, the expression of proliferation markers and the expression of glycolysis-related proteins. A, B Detection of PTPRH expression by immunohistochemistry (×400). C Correlation of PTPRH expression with 18F-FDG accumulation and the expression levels of Ki67. D In patients with high levels of PTPRH expression, immunohistochemical staining revealed high expression levels of GLUT1, HK2, PKM2, and LDHA (×400), whereas patients with low levels of PTPRH expression expressed low levels of GLUT1, HK2, PKM2 and LDHA (× 400). E The expression levels of PTPRH correlated positively with SUVmax, MTV, and TLG values and the expression of Ki67, GLUT1, HK2, PKM2, LDHA. 18F-FDG: 18F-fluorodeoxyglucose; GLUT1: glucose transporter type 1; HK2: hexokinase 2; PKM2: pyruvate kinase M2; LDHA: lactate dehydrogenase A; SUVmax: maximum standard uptake value Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37974250), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PTPRH Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PTPRH

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracytoplasmic catalytic domain, and thus represents a receptor-type PTP. The extracellular region contains eight fibronectin type III-like repeats and multiple N-glycosylation sites. The gene was shown to be expressed primarily in brain and liver, and at a lower level in heart and stomach. It was also found to be expressed in several cancer cell lines, but not in the corresponding normal tissues. [provided by RefSeq]

Alternate Names

EC 3.1.3.48, FLJ39938, MGC133058, MGC133059, protein tyrosine phosphatase, receptor type, H, receptor-type tyrosine-protein phosphatase H, R-PTP-H, SAP1, SAP-1, Stomach cancer-associated protein tyrosine phosphatase 1, Transmembrane-type protein-tyrosine phosphatase type H

Gene Symbol

PTPRH

UniProt

Additional PTPRH Products

Product Documents for PTPRH Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PTPRH Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for PTPRH Antibody - BSA Free

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Protocols

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