PTPRH Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-20005
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Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human PTPRH. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%)
Localization
Membrane; Single-pass type I membrane protein (Potential); Cytoplasm
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
122 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for PTPRH Antibody - BSA Free
Western Blot: PTPRH Antibody [NBP2-20005]
Western Blot: PTPRH Antibody [NBP2-20005] - Sample (30 ug of whole cell lysate) A: HeLa 7. 5% SDS PAGE gel, diluted at 1:1000.Immunocytochemistry/ Immunofluorescence: PTPRH Antibody [NBP2-20005]
Immunocytochemistry/Immunofluorescence: PTPRH Antibody [NBP2-20005] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.Immunohistochemistry-Paraffin: PTPRH Antibody [NBP2-20005] -
PTPRH antibody [C2C3], C-term detects PTPRH protein at cytoplasm in rat colon by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.
PTPRH antibody [C2C3], C-term (NBP2-20005) diluted at 1:500.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: PTPRH Antibody - BSA Free [NBP2-20005] -
PTPRH promoted proliferation in NSCLC. A, B Detection of PTPRH expression using RT‒PCR and western blotting. C, D Expression of PTPRH after transfection by western blotting analysis and RT‒PCR. E The results of the colony formation assay.) F 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. G, H 5-ethynyl-2′-deoxyuridine (EdU) assay show that PTPRH enhances the proliferation ability of NSCLC cells Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37974250), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Knockdown Validated: PTPRH Antibody - BSA Free [NBP2-20005] -
PTPRH promoted proliferation in NSCLC. A, B Detection of PTPRH expression using RT‒PCR and western blotting. C, D Expression of PTPRH after transfection by western blotting analysis and RT‒PCR. E The results of the colony formation assay.) F 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. G, H 5-ethynyl-2′-deoxyuridine (EdU) assay show that PTPRH enhances the proliferation ability of NSCLC cells Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37974250), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Knockdown Validated: PTPRH Antibody - BSA Free [NBP2-20005] -
PTPRH inhibited cell cycle arrest and apoptosis. A, B Gene Set Enrichment Analysis (GSEA) of PTPRH. C, D Flow cytometry analysis of the apoptotic effect of PTPRH on NSCLC and statistical analysis. E, F Flow cytometry analysis of the effects of PTPRH expression on the cell cycle in NSCLC and statistical analysis. G Western blotting results showing the effects of short hairpin RNA-PTPRH (sh-PTPRH) treatment on cell cycle-related proteins. H Western blotting results showing the effects of sh-PTPRH treatment on the expression of apoptotic proteins Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37974250), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Flow Cytometry: PTPRH Antibody - BSA Free [NBP2-20005] -
Correlation of PTPRH expression with 18F-fluorodeoxyglucose (18F-FDG) semiquantitative indicators, the expression of proliferation markers and the expression of glycolysis-related proteins. A, B Detection of PTPRH expression by immunohistochemistry (×400). C Correlation of PTPRH expression with 18F-FDG accumulation and the expression levels of Ki67. D In patients with high levels of PTPRH expression, immunohistochemical staining revealed high expression levels of GLUT1, HK2, PKM2, and LDHA (×400), whereas patients with low levels of PTPRH expression expressed low levels of GLUT1, HK2, PKM2 and LDHA (× 400). E The expression levels of PTPRH correlated positively with SUVmax, MTV, and TLG values and the expression of Ki67, GLUT1, HK2, PKM2, LDHA. 18F-FDG: 18F-fluorodeoxyglucose; GLUT1: glucose transporter type 1; HK2: hexokinase 2; PKM2: pyruvate kinase M2; LDHA: lactate dehydrogenase A; SUVmax: maximum standard uptake value Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37974250), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for PTPRH Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PTPRH
Alternate Names
EC 3.1.3.48, FLJ39938, MGC133058, MGC133059, protein tyrosine phosphatase, receptor type, H, receptor-type tyrosine-protein phosphatase H, R-PTP-H, SAP1, SAP-1, Stomach cancer-associated protein tyrosine phosphatase 1, Transmembrane-type protein-tyrosine phosphatase type H
Gene Symbol
PTPRH
UniProt
Additional PTPRH Products
Product Documents for PTPRH Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PTPRH Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.Citations for PTPRH Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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