![Western Blot: PUM3 Antibody [NBP1-87296]](https://resources.rndsystems.com/images/products/KIAA0020-Antibody-Western-Blot-NBP1-87296-img0009.jpg "Western Blot: PUM3 Antibody [NBP1-87296]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EHAQEVVLDKSACVLVSDILGSATGDVQPTMNAIASLAATGLHPGGKDGELHIAEHPAGHLVLKWLIEQDKKMKENGREGCFAKTLVEHVGMKNLKSWASVNRGAIILSSLLQSCDLEVANKVKAALKSLIPTLEKTKSTSKGI
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (85%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PUM3 Antibody - BSA Free
Western Blot: PUM3 Antibody [NBP1-87296]
Western Blot: KIAA0020 Antibody [NBP1-87296] - Analysis in control (vector only transfected HEK293T lysate) and KIAA0020 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: PUM3 Antibody [NBP1-87296]](https://resources.rndsystems.com/images/products/KIAA0020-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87296-img0008.jpg "Immunocytochemistry/ Immunofluorescence: PUM3 Antibody [NBP1-87296]")
Immunocytochemistry/ Immunofluorescence: PUM3 Antibody [NBP1-87296]
Immunocytochemistry/Immunofluorescence: KIAA0020 Antibody [NBP1-87296] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoli. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]](https://resources.rndsystems.com/images/products/KIAA0020-Antibody-Immunohistochemistry-Paraffin-NBP1-87296-img0012.jpg "Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]")
Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]
Immunohistochemistry-Paraffin: KIAA0020 Antibody [NBP1-87296] - Staining of human colon shows weak to moderate positivity in nucleoli in glandular cells.![Western Blot: PUM3 Antibody [NBP1-87296]](https://resources.rndsystems.com/images/products/KIAA0020-Antibody-Western-Blot-NBP1-87296-img0007.jpg "Western Blot: PUM3 Antibody [NBP1-87296]")
Western Blot: PUM3 Antibody [NBP1-87296]
Western Blot: KIAA0020 Antibody [NBP1-87296] - Analysis in human cell line HDLM-2.![Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]](https://resources.rndsystems.com/images/products/KIAA0020-Antibody-Immunohistochemistry-Paraffin-NBP1-87296-img0002.jpg "Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]")
Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]
Immunohistochemistry-Paraffin: KIAA0020 Antibody [NBP1-87296] - Staining of human breast shows nuclear membranous positivity in glandular cells.![Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]](https://resources.rndsystems.com/images/products/KIAA0020-Antibody-Immunohistochemistry-Paraffin-NBP1-87296-img0010.jpg "Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]")
Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]
Immunohistochemistry-Paraffin: KIAA0020 Antibody [NBP1-87296] - Staining of human kidney shows weak to moderate positivity in nucleoli in cells in tubules.![Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]](https://resources.rndsystems.com/images/products/KIAA0020-Antibody-Immunohistochemistry-Paraffin-NBP1-87296-img0011.jpg "Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]")
Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]
Immunohistochemistry-Paraffin: KIAA0020 Antibody [NBP1-87296] - Staining of human testis shows weak to moderate positivity in nucleoli in cells in seminiferous ducts.![Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]](https://resources.rndsystems.com/images/products/KIAA0020-Antibody-Immunohistochemistry-Paraffin-NBP1-87296-img0013.jpg "Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]")
Immunohistochemistry-Paraffin: PUM3 Antibody [NBP1-87296]
Immunohistochemistry-Paraffin: KIAA0020 Antibody [NBP1-87296] - Staining of human fallopian tube shows weak to moderate positivity in nucleoli in glandular cells.Applications for PUM3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:20 - 1:50
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: PUM3
Alternate Names
HBV X-transactivated gene 5 protein, HLA-HA8MGC8749, KIAA0020, KIAA0020 protein, Minor histocompatibility antigen HA-8, PEN, penguin homolog, protein 5 transactivated by hepatitis B virus X antigen (HBxAg), PUF6, XTP5HBV XAg-transactivated protein 5
Gene Symbol
KIAA0020
Additional PUM3 Products
Product Documents for PUM3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PUM3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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