PUS10 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-48941

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PUS10 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: NSPKAVCAVLEIECAHGAVFVAGRYNKYSRNLPQTPWIIDGERKLESSVEELISDHLLAVFKAESFNFSSSGREDVDVRTLGNGRPFAIELVNPHRVHFTSQEIKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PUS10 Antibody - BSA Free

Western Blot: PUS10 Antibody [NBP2-48941]

Western Blot: PUS10 Antibody [NBP2-48941]

Western Blot: PUS10 Antibody [NBP2-48941] - Analysis in human cell line HepG2.
Immunocytochemistry/ Immunofluorescence: PUS10 Antibody [NBP2-48941]

Immunocytochemistry/ Immunofluorescence: PUS10 Antibody [NBP2-48941]

Immunocytochemistry/Immunofluorescence: PUS10 Antibody [NBP2-48941] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & mitochondria.
Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941]

Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941]

Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941] - Staining of human Cerebral cortex shows moderate nuclear and cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941]

Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941]

Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941] - Staining of human thyroid gland shows moderate nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941]

Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941]

Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941] - Staining of human testis shows moderate nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941]

Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941]

Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941] - Staining of human tonsil shows moderate nuclear and cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941]

Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941]

Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941] - Staining of human cerebral cortex shows moderate nuclear and cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941]

Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941]

Immunohistochemistry-Paraffin: PUS10 Antibody [NBP2-48941] - Staining of human stomach shows moderate nuclear and ctyoplasmic positivity in glandular cells.
PUS10 Antibody - BSA Free

Immunohistochemistry: PUS10 Antibody - BSA Free [NBP2-48941] -

PUS10 inhibits the migration of RCC in a catalytic-independent manner. A Overexpression of PUS10 was verified by qRT‒PCR using GAPDH as a reference. B Western blot and dot blot assay were performed to verify the construction of wildtype and mutant PUS10 overexpressing models in vitro. C Transwell assays were conducted to evaluate the migration inhibition effect of wild-type and catalytically incompetent PUS10. Migrating cells in three replicate experiments were counted and are presented as the mean +/- SD in the histogram. D Wound healing assay validates the similar impact of wild-type and mutant PUS10 ectopic expression on the migratory ability of RCC cells. E Transwell assays showed that supplementation with either wild-type or mutant PUS10 could reverse the promotion of RCC migration induced by PUS10 silencing with siRNA. Migrating cells in three replicate experiments were counted and are presented as the mean +/- SD in the histogram. *P < 0.05, **P < 0.01, ***P < 0.001; ns not significant Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37596681), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for PUS10 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PUS10

Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotidemodification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylatesynthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicingRNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty etal., 2007 (PubMed 17900615)).(supplied by OMIM)

Alternate Names

CCDC139, coiled-coil domain containing 139, Coiled-coil domain-containing protein 139, DOBIMGC126729, EC 5.4.99, EC 5.4.99.-, FLJ32312, MGC126755, pseudouridine synthase 10, pseudouridylate synthase 10, Psi55 synthase, PUS1, putative tRNA pseudouridine synthase Pus10, tRNA pseudouridine 55 synthase, tRNA pseudouridylate synthase, tRNA-uridine isomerase

Gene Symbol

PUS10

Additional PUS10 Products

Product Documents for PUS10 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PUS10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PUS10 Antibody - BSA Free

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Protocols

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FAQs

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