QKI/Quaking Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20029

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Feline (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all QKI/Quaking Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human QKI/Quaking. The exact sequence is proprietary.

Localization

Nucleus (By similarity); Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for QKI/Quaking Antibody - BSA Free

Western Blot: QKI/Quaking Antibody [NBP2-20029]

Western Blot: QKI/Quaking Antibody [NBP2-20029]

Western Blot: QKI/Quaking Antibody [NBP2-20029] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody diluted at 1:1000.
Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029]

Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029]

Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: QKI protein stained by QKI antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
Western Blot: QKI/Quaking Antibody [NBP2-20029]

Western Blot: QKI/Quaking Antibody [NBP2-20029]

Western Blot: QKI/Quaking Antibody [NBP2-20029] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP2-20029]

Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP2-20029]

Immunohistochemistry-Paraffin: QKI/Quaking Antibody [NBP2-20029] - Immunohistochemical analysis of paraffin-embedded MDA-MB157 xenograft, using antibody at 1:100 dilution.
Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029]

Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029]

Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: QKI protein stained by QKI antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.
QKI/Quaking Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: QKI/Quaking Antibody [NBP2-20029] -

QKI antibody [N2C3] detects QKI protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: QKI protein stained by QKI antibody [N2C3] (NBP2-20029) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for QKI/Quaking Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: QKI/Quaking

QKI belongs to a family of RNA-binding proteins that have an HNRNPK (MIM 600712) homology (KH) domain embedded in a 200-amino acid region called the GSG domain. Other members of this family include SAM68 (KHDRBS1; MIM 602489) and SF1 (MIM 601516) (Chen and Richard, 1998 [PubMed 9671495]). QKI proteins regulate RNA splicing, export of target RNAs from the nucleus, translation of proteins, and RNA stability (Lauriat et al., 2008 [PubMed 17918747]).[supplied by OMIM]

Alternate Names

DKFZp586I0923, HKQ, homolog of mouse quaking QKI (KH domain RNA binding protein), Hqk, HqkI, protein quaking, QK, QK1, QK3, quaking homolog, KH domain RNA binding (mouse), RNA binding protein HQK

Gene Symbol

QKI

UniProt

Q96PU8 (Human)

Additional QKI/Quaking Products

Product Documents for QKI/Quaking Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for QKI/Quaking Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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