RAB11B Antibody - BSA Free

Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085]

Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Human colon carcinoma, using RAB11B antibody antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085]

Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Rat brain. RAB11B antibody [N1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085]

Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Mouse brain. RAB11B stained by RAB11B antibody [N1C3] diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085]

Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - Rat brain. RAB11B stained by RAB11B antibody [N1C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: RAB11B Antibody [NBP2-15085] -

Western Blot: RAB11B Antibody [NBP2-15085] - Proteomic profiling of autophagic cargo during NMDAR-LTD.a–d WB analyses of different fractions along the autophagic vesicle purification procedure, using antibodies against a autophagosomal markers (LC3B, p62, Atg16L1, & Atg9A), b ER-Golgi markers (TGN, LMAN1, SAR1a), c endosomal markers (Rab11b, EEA1), & d markers of plasma-membrane (Stx4), extracellular vesicles (Alix) & nuclear extracts (TBP). N = 3 independent experiments. e Graph showing the cell component analysis, as false discovery rate (FDR)-corrected p-values, of dynamic cargo (total of 393 proteins) that is enriched (up) or less abundant (down) in AVs after LTD, compared to control. f Graphical representation of proteins enriched in AVs upon LTD, w/ relation to synapse. g WB analysis of PK-treated control & LTD-AVs, validating the presence of proteins identified by the proteomic analyses in autophagic vesicles. Postsynaptic density (PSD) fraction used as reference. Graph showing the fold change of normalized levels of proteins validated by WB, as a ratio of LTD to control. Cargo proteins normalized to levels of p62, which remains unaffected at the early phase of LTD. N = 3 independent AV preparations. Bars represent mean values ± SEM. Statistical analysis performed using paired, two-tailed Student’s t-test (GluA1,N = 6, P = 0.0002; GluA2, N = 6, P = 0.0039; Pick1, N = 5, P = 0.011; SAP97, N = 5, P = 0.0179; FYN, N = 8, P < 0.0001; CamKIIa, N = 8, P < 0.0001; IL1RAPL1, N = 8, P = 0.0004; Adam22, N = 4, P = 0.0018; INA, N = 3, P = 0.0287; MYH10, N = 8, P < 0.0001; ITPKA, N = 6, P = 0.0006; KCC2, N = 4, P = 0. 0352; cofilin-1, N = 6, P = 0.005; dynamin, N = 6, P = 0.0005; p62, N = 6, P = 0.9809). All indicated molecular weights in a–d & g are in kDaltons (kD). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35115539), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] -

Immunohistochemistry-Paraffin: RAB11B Antibody [NBP2-15085] - RAB11B antibody [N1C3] detects RAB11B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
RAB11B stained by RAB11B antibody [N1C3] (NBP2-15085) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: RAB11B Antibody [NBP2-15085] -

Western Blot: RAB11B Antibody [NBP2-15085] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RAB11B antibody [N1C3] (NBP2-15085) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: RAB11B Antibody [NBP2-15085] -

RAB11B antibody [N1C3] detects RAB11B protein by immunofluorescent analysis.
Sample: DIV9 rat cortical neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RAB11B stained by RAB11B antibody [N1C3] (NBP2-15085) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.

Immunohistochemistry-Frozen: RAB11B Antibody [NBP2-15085] -

RAB11B antibody [N1C3] detects RAB11B Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: RAB11B stained by RAB11B antibody [N1C3] (NBP2-15085) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500.
Blue: Fluoroshield with DAPI.
br>Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Immunocytochemistry/ Immunofluorescence: RAB11B Antibody [NBP2-15085] -

GRAMD1B antibody [C1C2], Internal detects GRAMD1B protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde at RT for 15 min.
Green: GRAMD1B protein stained by GRAMD1B antibody [C1C2], Internal (NBP2-15085) diluted at 1:500.
Blue: Hoechst 33342 staining.