RAB9A Antibody (Clone 9) - Chimeric - BSA Free

Novus Biologicals | Catalog # NBP3-52358

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Hamster, Primate

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # Clone 9

Format

BSA Free
View all RAB9A Primary Antibodies »

Product Specifications

Immunogen

Recombinant canine RAB9A

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone 9 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone Rab9, clone 9 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.

Scientific Data Images for RAB9A Antibody (Clone 9) - Chimeric - BSA Free

Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (Clone 9) - Chimeric - BSA Free [NBP3-52358]

Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (Clone 9) - Chimeric - BSA Free [NBP3-52358]

Immunofluorescent staining of HeLa cells using NBP3-52358 (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the late endosomes in HeLa cells.
Western Blot: RAB9A Antibody (Clone 9) - Chimeric - BSA Free [NBP3-52358]

Western Blot: RAB9A Antibody (Clone 9) - Chimeric - BSA Free [NBP3-52358]

Western blotting of HeLa cell lysates with NBP3-52358 at ~23 kDa. The antibody was detected with anti-chicken-HRP.
Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (Clone 9) - Chimeric - BSA Free [NBP3-52358]

Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (Clone 9) - Chimeric - BSA Free [NBP3-52358]

Immunofluorescent staining of HeLa cells using NBP3-52358 (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the late endosomes in HeLa cells.
Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (Clone 9) - Chimeric - BSA Free [NBP3-52358]

Immunocytochemistry/ Immunofluorescence: RAB9A Antibody (Clone 9) - Chimeric - BSA Free [NBP3-52358]

Immunofluorescent staining of HeLa cells using NBP3-52358 (green). Actin filaments were stained using Phalloidin (red). DAPI nuclear stain (blue) shows cell nuclei. This antibody specifically stains the late endosomes in HeLa cells.

Applications for RAB9A Antibody (Clone 9) - Chimeric - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

1X PBS (pH 7.4)

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAB9A

Rab proteins are a family of Ras-like GTPases involved in intracellular compartment protein transport. Different members of the 40+ member rab family are responsible for docking and fusion of transport vesicles between different compartments within the cell. Rab 9 is required for trafficking mannose 6-phosphate receptor between the late endosome to trans-Golgi network (TGN). By facilitating receptor transport, rab 9 enables cells to efficiently recycle important cellular trafficking components. It is functionally necessary for rab 9, like other rab family members, to be prenylated by two 20-carbon geranylgeranyl groups at the C-terminus. Most prenylated rab 9 is membrane bound, however, 10-20% of the cellular pool of rab 9 is bound to GDP dissociation inhibitor-alpha (GDI-alpha) in the cytosol. GDI recycles prenylated, GDP bound rab 9 from their fusion targets back to their membranes of origin.

Alternate Names

RAB9A, member RAS oncogene family, RAB9RAB9, member RAS oncogene family, ras-related protein Rab-9A

Gene Symbol

RAB9A

Additional RAB9A Products

Product Documents for RAB9A Antibody (Clone 9) - Chimeric - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RAB9A Antibody (Clone 9) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RAB9A Antibody (Clone 9) - Chimeric - BSA Free

There are currently no reviews for this product. Be the first to review RAB9A Antibody (Clone 9) - Chimeric - BSA Free and earn rewards!

Have you used RAB9A Antibody (Clone 9) - Chimeric - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies