Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 699811
View all VAP-B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat VAP-B
Ala2-Pro132
Accession # Q9Z269

Specificity

Detects rat VAP-B in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) VAP-B and approximately 50% cross-reactivity with rhVAP-A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Rat VAP‑B Antibody

Detection of Rat VAP-B antibody by Western Blot.

Detection of Rat VAP‑B by Western Blot.

Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Rat VAP-B Monoclonal Antibody (Catalog # MAB7329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAP-B at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

VAP-B antibody in Rat Brain by Immunohistochemistry (IHC-Fr).

VAP‑B in Rat Brain.

VAP-B was detected in perfusion fixed frozen sections of rat brain (medulla) using Mouse Anti-Rat VAP-B Monoclonal Antibody (Catalog # MAB7329) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to gigantocellular neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Rat VAP‑B Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (medulla)

Western Blot

0.5 µg/mL
Sample: PC‑12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VAP-B

Vesicle-associated membrane protein (VAMP)-associated protein B (VAP-B; also VAMP-B) is an ~30 kDa ubiquitously expressed type IV transmembrane protein belonging to the VAP family. It is found in endoplasmic reticulum (ER), Golgi and other membranes as a homodimer or a heterodimer with VAP-A, probably associating through the transmembrane regions. Human VAP-B cDNA encodes a 222 amino acids (aa) cytoplasmic domain and a 21 aa C-terminal membrane anchor. Rat VAP-B shares 89% and 96% aa identity with mouse and human VAP-B, respectively. A soluble form can function as a ligand for EPH receptors. A human polymorphism of VAP-B, P56S, is found in three familial motor neuron diseases, notably the amylotrophic lateral sclerosis variant ALS8.

Long Name

VAMP [Vesicle-associated Membrane Protein]-associated Protein B and C

Alternate Names

ALS8, VAMP-B, VAMP-C, VAP-C, VAPB

Entrez Gene IDs

9217 (Human); 56491 (Mouse); 60431 (Rat)

Gene Symbol

VAPB

UniProt

Q9Z269

Additional VAP-B Products

Product Documents for Rat VAP‑B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat VAP‑B Antibody

For research use only

Citations for Rat VAP‑B Antibody

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Protocols

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