Rat VAP-B Antibody (699811)

R&D Systems | Catalog # MAB7329

R&D Systems

Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 699811
View all VAP-B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat VAP-B
Ala2-Pro132
Accession # Q9Z269

Specificity

Detects rat VAP-B in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) VAP-B and approximately 50% cross-reactivity with rhVAP-A is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Rat VAP-B Antibody (699811)

Detection of Rat VAP-B antibody by Western Blot.

Detection of Rat VAP‑B by Western Blot.

Western blot shows lysates of PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Rat VAP-B Monoclonal Antibody (Catalog # MAB7329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAP-B at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
VAP-B antibody in Rat Brain by Immunohistochemistry (IHC-Fr).

VAP‑B in Rat Brain.

VAP-B was detected in perfusion fixed frozen sections of rat brain (medulla) using Mouse Anti-Rat VAP-B Monoclonal Antibody (Catalog # MAB7329) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to gigantocellular neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Rat VAP-B Antibody (699811)

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (medulla)

Western Blot

0.5 µg/mL
Sample: PC‑12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VAP-B

Vesicle-associated membrane protein (VAMP)-associated protein B (VAP-B; also VAMP-B) is an ~30 kDa ubiquitously expressed type IV transmembrane protein belonging to the VAP family. It is found in endoplasmic reticulum (ER), Golgi and other membranes as a homodimer or a heterodimer with VAP-A, probably associating through the transmembrane regions. Human VAP-B cDNA encodes a 222 amino acids (aa) cytoplasmic domain and a 21 aa C-terminal membrane anchor. Rat VAP-B shares 89% and 96% aa identity with mouse and human VAP-B, respectively. A soluble form can function as a ligand for EPH receptors. A human polymorphism of VAP-B, P56S, is found in three familial motor neuron diseases, notably the amylotrophic lateral sclerosis variant ALS8.

Long Name

VAMP [Vesicle-associated Membrane Protein]-associated Protein B and C

Alternate Names

ALS8, VAMP-B, VAMP-C, VAP-C, VAPB

Entrez Gene IDs

9217 (Human); 56491 (Mouse); 60431 (Rat)

Gene Symbol

VAPB

UniProt

Q9Z269

Additional VAP-B Products

Product Documents for Rat VAP-B Antibody (699811)

Certificate of Analysis

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Product Specific Notices for Rat VAP-B Antibody (699811)

For research use only

Citations for Rat VAP-B Antibody (699811)

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Protocols

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