Potassium Channel Kv2.2 Antibody
R&D Systems | Catalog # PPS015
Voltage Gated
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Discontinued Product
PPS015 has been discontinued.
View all Potassium Channel Kv2.2 products.
Key Product Details
Species Reactivity
Rat, Xenopus
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Peptide from the Kv2.2 subunit region of Xenopus and rodent, conjugated to keyhole limpet hemocyanin (KLH)
Specificity
Rodent and Xenopus ~125 kDa Kv2.2 subunit of the Voltage Gated Potassium Channel protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Potassium Channel Kv2.2 Antibody
Detection of Potassium Channel Kv2.2 by Western Blot
Western blot of rat brain homogenate. As shown in the autoradiograph, the antibody is specific for the ~125 kDa Voltage Gated Potassium Channel, Kv2.2 subunit. The labeling is blocked by preadsorption with the immunizing peptide.Applications for Potassium Channel Kv2.2 Antibody
Application
Recommended Usage
Immunohistochemistry
1:1000 dilution
Sample: Frozen sections; unpublished observations
Sample: Frozen sections; unpublished observations
Western Blot
1:1000 dilution
Sample: Rat brain tissue
Sample: Rat brain tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.
Background: Potassium Channel Kv2.2
References
- Trimmer, J.S. and K.J. Rhodes (2004) Annu. Rev. Physiol. 66:477.
- Malin, S.A. and J.M. Nerbonne (2002) J. Neurosci. 22:10094.
- Blaine, J.T. and A.B. Ribera (1998) J. Neurosci. 18:9585.
- Hwang, P.M. et al. (1992) Neuron 8:473.
- O’Grady, S.M. and S.Y. Lee (2005) Int. J. Biochem. Cell Biol. 37:1578.
Alternate Names
KCNB2
Gene Symbol
KCNB2
Additional Potassium Channel Kv2.2 Products
Product Documents for Potassium Channel Kv2.2 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Potassium Channel Kv2.2 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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