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Recombinant Human C1qR1/CD93 (24-580) His Avi Protein, CF

Catalog #: AVI11656 Datasheet / COA / SDS
His-tag Avi-tag
Catalog # Availability Size / Price Qty
AVI11656-050

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Biotinylated Recombinant Human C1qR1/CD93 (24-580) His-tag Avi-tag Protein Binding Activity.
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Recombinant Human C1qR1/CD93 (24-580) His Avi Protein, CF Summary

  • R&D Systems HEK293-derived Recombinant Human C1qR1/CD93 (24-580) His Avi Protein (AVI11656)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Product Specifications

Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human C1qR1/CD93 (24-580) His-tag Avi-tag binds to Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein (Catalog # 1334-B7) with an ED50 of 1.50-15.0 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived human C1qR1/CD93 protein
Ala24-Lys580, with a C-terminal 6-His tag and Avi-tag
Accession #
Q9NPY3.3
N-terminal Sequence
Analysis
Ala24
Structure / Form
Biotinylated via Avi-tag
Predicted Molecular Mass
61 kDa
SDS-PAGE
82-105 kDa, under reducing conditions.

Product Datasheets

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AVI11656

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

AVI11656

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity View Larger

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human C1qR1/CD93 (24-580) His-tag Avi-tag Protein (Catalog # AVI11656) binds to Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein (1334-B7) with an ED50 of 1.50‑15.0 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Biotinylated Recombinant Human C1qR1/CD93 (24-580) His-tag Avi-tag Protein (Catalog # AVI11656) was resolved with SDS-PAGE under reducing (R) condition and visualized by Coomassie® Blue staining, showing bands at 82-105 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: C1qR1/CD93

C1qR1, also known as CD93 and C1qRp, is an approximately 125 kDa type-1 transmembrane glycoprotein that is involved in various aspects of inflammatory reactions (1). Mature human CD93 consists of a 557 amino acid (aa) extracellular domain (ECD) containing C‑type lectin and EGF‑like domains, followed by a 21 aa transmembrane segment and a 51 aa cytoplasmic domain (2, 3). Within the ECD, human CD93 shares 65% aa sequence identity with mouse and rat CD93. CD93 is expressed by vascular endothelial cells (5) and by a variety of hematopoietic cells (3‑9). Various sized fragments of soluble CD93 (50‑75 kDa) can be shed from monocytes, neutrophils, and vascular endothelial cells following inflammatory stimulation, leaving a residual stub in the membrane (11‑13). Cross‑linking of cell surface CD93 enhances phagocytosis by monocytes and enhances the uptake of apoptotic cells in vivo (10, 15). Soluble CD93 promotes the differentiation of monocytes to macrophages, phagocytosis of apoptotic cells, and inflammatory responsiveness to multiple TLR ligands (12, 14). CD93 plays a role in cardiovascular disease progression and modulates angiogenesis, inflammation and tumor growth and its interaction with insulin-like growth factor binding protein 7 (IGFBP7) contributes to abnormal tumor vasculature (16-17). Our Avi-tag Biotinylated Human C1qR1/CD93 (24-580) features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References
  1. Greenlee-Wacker, M.C. et al. (2012) Curr. Drug Targets 13:411.
  2. Nepomuceno, R.R. et al. (1997) Immunity 6:119.
  3. Steinberger, P. et al. (2002) J. Leukoc. Biol. 71:133.
  4. Nepomuceno, R.R. and A.J. Tenner (1998) J. Immunol. 160:1929.
  5. McGreal, E.P. et al. (2002) J. Immunol. 168:5222.
  6. Lovik, G. et al. (2001) Scand. J. Immunol. 53:410.
  7. Danet, G.H. et al. (2002) Proc. Natl. Acad. Sci. USA 99:10441.
  8. Ikewaki, N. et al. (2010) J. Clin. Immunol. 30:723.
  9. Chevrier, S. et al. (2009) Proc. Natl. Acad. Sci. USA 106:3895.
  10. Norsworthy, P.J. et al. (2004) J. Immunol. 172:3406.
  11. Bohlson, S.S. et al. (2005) J. Immunol. 175:1239.
  12. Greenlee, M.C. et al. (2009) Inflamm. Res. 58:909.
  13. Greenlee-Wacker, M.C. et al. (2011) J. Immunol. 187:3353.
  14. Jeon, J.W. et al. (2010) J. Immunol. 185:4921.
  15. Nepomuceno, R.R. et al. (1999) J. Immunol. 162:3583.
  16. Tossetta, G. et al. (2023) Cells 12:1778.
  17. Sun, Y. et al. (2021) Sci. Transl. Med. 13:eabc8922.
Long Name
Complement Component C1q Receptor
Entrez Gene IDs
22918 (Human); 17064 (Mouse); 84398 (Rat)
Alternate Names
AA4 Antigen; C1q R1; C1q receptor 1; C1q Rp; C1q/MBL/SPA receptor; C1qR(P); C1qR1; C1qRp; CD93 antigenC1qR; CD93 molecule; CD93; CDw93C1QR1; Collectin Receptor; Complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1; complement component C1q receptor; dJ737E23.1; ECSM3; Ly68; Matrix-remodeling-associated protein 4; matrix-remodelling associated 4; MXRA4

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