RhoA Antibody

Novus Biologicals | Catalog # NB100-91273

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RhoA Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from amino acid region 100-150 of human RhoA conjugated to blue carrier protein was used as the antigen. The peptide is homologous in many species including rat, mouse, chicken, dog, bovine, frog, zebrafish and orangutan.

Reactivity Notes

Marmoset

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RhoA Antibody

Western Blot: RhoA Antibody [NB100-91273]

Western Blot: RhoA Antibody [NB100-91273]

Western Blot: RhoA Antibody [NB100-91273] - Rat spinal cord lysate using Rabbit antibody to Transforming protein RhoA: whole serum at 1:2000 dilution. One single band is detected.
Immunocytochemistry/ Immunofluorescence: RhoA Antibody [NB100-91273]

Immunocytochemistry/ Immunofluorescence: RhoA Antibody [NB100-91273]

Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.
Immunohistochemistry: RhoA Antibody [NB100-91273]

Immunohistochemistry: RhoA Antibody [NB100-91273]

Immunohistochemistry: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin sections) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.
Immunocytochemistry/ Immunofluorescence: RhoA Antibody [NB100-91273]

Immunocytochemistry/ Immunofluorescence: RhoA Antibody [NB100-91273]

Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.
Immunocytochemistry/ Immunofluorescence: RhoA Antibody [NB100-91273]

Immunocytochemistry/ Immunofluorescence: RhoA Antibody [NB100-91273]

Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.
Immunocytochemistry/ Immunofluorescence: RhoA Antibody [NB100-91273]

Immunocytochemistry/ Immunofluorescence: RhoA Antibody [NB100-91273]

Immunocytochemistry/Immunofluorescence: RhoA Antibody [NB100-91273] - Rat trigeminal at 1:500 dilution using Rabbit antibody to Transforming protein RhoA: whole serum (NB100-91273, in red), Sheep antibody to extracellular, N-terminal part of Sortilin: whole serum (NB100-98771, in green), DAPI counter stained appearing in blue.
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273]

Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273]

Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273]

Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273]

Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273]

Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273]

Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - Rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273]

Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273]

Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on rat brain (paraffin section) using Rabbit antibody to Transforming protein RhoA (Arha, Arha2): whole serum at 1 : 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.
Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273]

Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273]

Immunohistochemistry-Paraffin: RhoA Antibody [NB100-91273] - IHC on mouse brain (cryo section) using Rabbit antibody to RhoA at a concentration of 15 ug/ml, incubated overnight and developed with DAB Ni.
RhoA Antibody

Western Blot: RhoA Antibody [NB100-91273] -

Melatonin injection restores renal cortical fibrosis in a CKD mouse model via increased expression of miR-4516. (A) Hematoxylin and eosin (H&E) staining was performed on kidney sections from a CKD mouse model following melatonin injection, or melatonin inhibition with miR-4516 inhibitor (scale bar = 1000 μm). (B,C) Expression of miR-4516 and ITGA9 was detected in the kidney cortex in each group by qPCR (n = 3). (D–F) Western blot analysis for ITGA9, Rac1, RhoA, CDC42, collagen type 1, and fibronectin expression using samples from the kidney cortex of each mouse model group (n = 3). Protein levels were determined by densitometry relative to alpha -tubulin. The values represent mean +/- SEM. * p < 0.05, ** p < 0.01 vs. healthy kidney, ##p < 0.01 vs. phosphate buffered saline (PBS), $$p < 0.01 vs. melatonin. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32727098), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
RhoA Antibody

Western Blot: RhoA Antibody [NB100-91273] -

Cytoskeleton reorganization and ITGA9-Rho GTPase signaling pathways are activated due to decreased miR-4516 expression following P-cresol exposure. (A,B) Expression of miR-4516 and ITGA9 was detected in human proximal tubular epithelial (TH1) cells with P-cresol (0.1, 0.25, and 0.5 mM) or indoxyl sulfate (0.2, 0.4, and 0.8 mM) exposure for 72 h (n = 3). The values represent mean +/- SEM. * p < 0.05, ** p < 0.01 vs. control. (C,D) Western blot analysis for ITGA9, Rac1, RhoA, and CDC42 in TH1 cells after exposure to various doses of P-cresol (0, 0.1, 0.25, and 0.5 mM) for 72 h (n = 3). Protein expression was determined by densitometry relative to beta -actin. The values represent mean +/- SEM. ** p < 0.01 vs. control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32727098), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RhoA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:500

Immunohistochemistry

1:1000

Immunohistochemistry-Paraffin

1:1000

Western Blot

1:1000

Reviewed Applications

Read 1 review rated 3 using NB100-91273 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RhoA

RhoA belongs to the small GTPase superfamily, Rho family. It regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesion and actin stress fibers. It serves as a target for the yopT cysteine peptidase from Yersinia pestis, vector of the plague, and Yersinia pseudotuberculosis, which causes gastrointestinal disorders. It binds PRKCL1, ROCK1 and ROCK2. Interacts with NET1 and ARHGEF3.

Alternate Names

h12, oncogene RHO H12, ras homolog gene family, member A, Rho cDNA clone 12, Rho12, RhoA, RHOH12ARH12ARHAAplysia ras-related homolog 12, small GTP binding protein RhoA, transforming protein RhoA

Entrez Gene IDs

11848 (Mouse); 117273 (Rat)

Gene Symbol

RHOA

UniProt

P61586 (Human)

Additional RhoA Products

Product Documents for RhoA Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RhoA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RhoA Antibody (1)

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  • RhoA Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse embryonic fibroblast cell line, Sample Amount: 40 ug
    Species: Mouse
    Verified Customer | Posted 05/09/2017
    Testing antibody from Novus for RhoA (seen in white dashed box). Perhaps too much protein (40ug) was loaded. Band location was as expected (~25kDa).
    MEFs cultured in 10% FBS in DMEM for 48 hours
    RhoA Antibody NB100-91273

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Protocols

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