RHOT2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88982

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RHOT2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: RSCLGHLGYLGYPTLCEQDQAHAITVTREKRLDQEKGQTQRSVLLCKVVGACGVGKSAFLQAFLGRGLGHQDTREQPPGYAIDTVQVNGQEKYLILC

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 24671417).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RHOT2 Antibody - BSA Free

Western Blot: RHOT2 Antibody [NBP1-88982]

Western Blot: RHOT2 Antibody [NBP1-88982]

Western Blot: RHOT2 Antibody [NBP1-88982] - Analysis in human cell line HEK 293.
Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982]

Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982]

Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982] - Staining of human Liver shows moderate granular cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982]

Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982]

Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982] - Staining of human Cerebral cortex shows moderate granular cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982]

Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982]

Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982] - Staining of human Heart muscle shows moderate granular cytoplasmic positivity in cardiomyocytes.
Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982]

Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982]

Immunohistochemistry-Paraffin: RHOT2 Antibody [NBP1-88982] - Staining of human Skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.
Simple Western: RHOT2 Antibody [NBP1-88982]

Simple Western: RHOT2 Antibody [NBP1-88982]

Simple Western: RHOT2 Antibody [NBP1-88982] - Simple Western lane view shows a specific band for RHOT2 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: RHOT2 Antibody [NBP1-88982]

Simple Western: RHOT2 Antibody [NBP1-88982]

Simple Western: RHOT2 Antibody [NBP1-88982] - Electropherogram image(s) of corresponding Simple Western lane view. RHOT2 antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).
RHOT2 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: RHOT2 Antibody [NBP1-88982]

Immunocytochemistry/ Immunofluorescence: RHOT2 Antibody [NBP1-88982]

Staining of human cell line U-2 OS shows localization to cytosol.

Applications for RHOT2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Simple Western

1:20

Western Blot

0.04-0.4 ug/ml
Application Notes
WB, ICC/IF reported in the literature (PMID:24671417). ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended. Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 75 kDa

Reviewed Applications

Read 1 review rated 5 using NBP1-88982 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RHOT2

RHOT2 is a gene that codes for a protein that is 618 amino acids long and weighs approximately 68 kDa and has a shorter isoform that is 213 amino acids long and weighs approximately 23 kDa. The protein that RHOT2 codes for is a mitochondrial GTPase that is involved in mitochondrial trafficking as well as the control of the subcellular distribution of mitochondria. Current research is being done on diseases and disorders linked to this gene including hypoxia. RHOT2 has been shown to have interactions with ZFYVE9, RYK, TRAK1, BECN1, and CLN3 in pathways such as the Rho GTPase cycle and signal transduction pathways.

Alternate Names

ARHT2, C16orf39, chromosome 16 open reading frame 39, EC 3.6.5, EC 3.6.5.-, hMiro-2, MIRO-2mitochondrial Rho 2, mitochondrial Rho GTPase 2, Ras homolog gene family member T2, ras homolog gene family, member T2, RASL

Gene Symbol

RHOT2

Additional RHOT2 Products

Product Documents for RHOT2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RHOT2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RHOT2 Antibody - BSA Free

Customer Reviews for RHOT2 Antibody - BSA Free (1)

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  • RHOT2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Tissue Lysates
    Species: Human
    Verified Customer | Posted 04/11/2019
    Western blot analysis of meningioma tumour (whole) tumour lysates yielded strong/clear bands at the predicted molecular weight. highly recommended Ab

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Protocols

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