Ribosomal Protein S6/RPS6 Antibody

Novus Biologicals | Catalog # NB100-1595

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Rat (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Ribosomal Protein S6/RPS6 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 200 and the C-terminus (residue 249) of human Ribosomal Protein S6 using the numbering given in entry NP_001001.2 (GeneID 6194).

Reactivity Notes

X. laevis (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ribosomal Protein S6/RPS6 Antibody

Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595]

Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595]

Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human and mouse RPS6 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RPS6 antibody NB100-1595 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.Previous
Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NB100-1595]

Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NB100-1595]

Immunohistochemistry-Paraffin: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-RPS6 (NB100-1595). Detection: DAB
Immunoprecipitation: Ribosomal Protein S6/RPS6 Antibody [NB100-1595]

Immunoprecipitation: Ribosomal Protein S6/RPS6 Antibody [NB100-1595]

Immunoprecipitation: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Detection of human RPS6 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RPS6 antibody NB100-1595 (lot NB100-1595-2) used for IP at 6 ug per reaction. RPS6 was also immunoprecipitated by a previous lot of this antibody (lot NB100-1595-1). For blotting immunoprecipitated RPS6, NB100-1595 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Ribosomal Protein S6/RPS6 Antibody

Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] -

Western Blot: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] - Loss of SPAG5 & CLUH lead to hyperactivation of mTORC1 signaling.(A, B) MSigDB Hallmark pathways of downregulated (E) or upregulated (F) proteins (with a cutoff of p ≤ 0.05; q ≤ 0.15) detected in proteomics analysis of SPAG5 ind-KO cells (Figure 4A, Supplementary file 3) using the EnrichR webtool. (C) Western blots of WT & SPAG5 ind-KO HeLa cells transfected with siRNA against CLUH or untargeted control siRNA. Cells were induced for 4 days with doxycycline, additionally downregulated for the last 3 days & grown for the last 8 hr in basal or HBSS media without doxycycline. Pan-actin was used as loading control. (D) Quantification of experiments as shown in C (n = 4 independent experiments). Antibody signal was normalized to pan-actin signal, & signal of phospho-protein was normalized to signal of the total protein. Bars show mean ± standard error of the mean (SEM) & dots represent values of individual replicates. One-way analysis of variance (ANOVA) with post hoc Tukey’s multiple comparison tests were performed with *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001.Figure 5—figure supplement 1—source data 1.Uncropped blots for Figure 5—figure supplement 1C.Figure 5—figure supplement 1—source data 2.Unedited blots for Figure 5—figure supplement 1C.Uncropped blots for Figure 5—figure supplement 1C.Unedited blots for Figure 5—figure supplement 1C. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35559794), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Ribosomal Protein S6/RPS6 Antibody

Immunocytochemistry/ Immunofluorescence: Ribosomal Protein S6/RPS6 Antibody [NB100-1595] -

L1 bodies (LBs) are large cytoplasmic compartments enriched in ORF1p, L1 mRNA, and ribosomes.(A–E) Electron micrographs exemplifying the formation of LBs in Mael-/- spermatocytes. Boxed areas in A and B identify small cytoplasmic aggregates magnified in A’ and B’, respectively. Boxed area in C identifies an intermediate size LB magnified in C’. D and E show more prominent LBs, partially surrounded by a bilayer membrane (red arrows). Nuc: nucleus. Cyto: cytoplasm. NE: nuclear envelope. (F) Immunofluorescence staining of L1 ORF1p on Mael-/- spermatocytes shows ORF1p accumulation in LBs (left panel, magenta). 3D reconstruction of LBs (magenta) and nuclei (blue) (middle and right panels); numbers [1–5] are used to label corresponding cells. Scale bars: 5 μm. (G) HCR RNA-FISH of L1 RNA (green) and immunofluorescence staining of L1 ORF1p (magenta) on Mael+/- and Mael-/- testis sections. Scale bars: 5 μm. (H) Double immunofluorescence staining of ribosomal protein S6 (RPS6, top two rows, green) or L28 (RPL28, bottom two rows, green) and L1 ORF1p (magenta) showing their colocalization in LBs. Dotted white lines mark seminiferous tubule boundaries. Scale bars: 15 μm. See also S1 Fig. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37307272), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Ribosomal Protein S6/RPS6 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 4 using NB100-1595 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Ribosomal Protein S6/RPS6

Ribosomal protein S6 (RPS6) is a component of the 40S ribosomal subunit. Ribosomes are composed of a small 40S subunit and a large 60S subunit that function to catalyze protein synthesis. RPS6 is phosphorylated by many cellular kinases including ribosomal protein S6 kinase that is regulated via the mammalian target of rapamycin (mTOR) pathway. RPS6 has been demonstrated to be important in regulating cell size, glucose homeostasis, and translational control of mRNA. Alternate names for RPS6 include 40S ribosomal protein S6, and phosphoprotein NP33.

Alternate Names

RPS6

Entrez Gene IDs

6194 (Human); 20104 (Mouse)

Gene Symbol

RPS6

UniProt

P62753

Additional Ribosomal Protein S6/RPS6 Products

Product Documents for Ribosomal Protein S6/RPS6 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ribosomal Protein S6/RPS6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons
Translation Regulation

Citations for Ribosomal Protein S6/RPS6 Antibody

Customer Reviews for Ribosomal Protein S6/RPS6 Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: HepG2
    Species: Human
    Verified Customer | Posted 09/18/2015
    RPS6 in HepG2 total cell lysate
    Ribosomal Protein S6/RPS6 Antibody NB100-1595

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