RNF40 Antibody

Novus Biologicals | Catalog # NBP2-20189

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RNF40 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human RNF40. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNF40 Antibody

Western Blot: RNF40 Antibody [NBP2-20189]

Western Blot: RNF40 Antibody [NBP2-20189]

Western Blot: RNF40 Antibody [NBP2-20189] - RNF40 antibody detects RNF40 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RNF40 antibody diluted by 1:1000.
Immunohistochemistry-Paraffin: RNF40 Antibody [NBP2-20189]

Immunohistochemistry-Paraffin: RNF40 Antibody [NBP2-20189]

Immunohistochemistry-Paraffin: RNF40 Antibody [NBP2-20189] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.
Western Blot: RNF40 Antibody [NBP2-20189]

Western Blot: RNF40 Antibody [NBP2-20189]

Western Blot: RNF40 Antibody [NBP2-20189] - Sample (30 ug of whole cell lysate) A: IMR32 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunoprecipitation: RNF40 Antibody [NBP2-20189]

Immunoprecipitation: RNF40 Antibody [NBP2-20189]

Immunoprecipitation: RNF40 Antibody [NBP2-20189] - Immunoprecipitation of RNF40 protein from HeLa whole cell extracts using 5 ug of RNF40 antibody. Western blot analysis was performed using RNF40 antibody.

Applications for RNF40 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RNF40

The protein encoded by this gene contains a RING finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein was reported to interact with the tumor suppressor protein RB1. Studies of the rat counterpart suggested that this protein may function as an E3 ubiquitin-protein ligase, and facilitate the ubiquitination and degradation of syntaxin 1, which is an essential component of the neurotransmitter release machinery. [provided by RefSeq]

Alternate Names

95 kDa retinoblastoma-associated protein, BRE1-B, BRE1BMGC13051, EC 6.3.2,95 kDa retinoblastoma protein binding protein, EC 6.3.2.-, KIAA0661BRE1 E3 ubiquitin ligase homolog B, Rb-associated protein, RBP95E3 ubiquitin-protein ligase BRE1B, ring finger protein 40DKFZp686K191, STARING

Gene Symbol

RNF40

UniProt

O75150 (Human)

Additional RNF40 Products

Product Documents for RNF40 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RNF40 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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