RNF8 Antibody

Novus Biologicals | Catalog # NB100-1176

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RNF8 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence GEPGFFVTGDRAG-C corresponding to N-Terminus according to NP_003949.1, NP_898901.1.

Specificity

This antibody is expected to recognize both reported isoforms (NP_003949.1 and NP_898901.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNF8 Antibody

Western Blot: RNF8 Antibody [NB100-1176]

Western Blot: RNF8 Antibody [NB100-1176]

Western Blot: RNF8 Antibody [NB100-1176] - Staining of HepG2 (A) and Jurkat (B) nuclear cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Flow Cytometry: RNF8 Antibody [NB100-1176]

Flow Cytometry: RNF8 Antibody [NB100-1176]

Flow Cytometry: RNF8 Antibody [NB100-1176] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for RNF8 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Peptide ELISA

Detection limit 1:128000

Western Blot

0.5 - 2 ug/mL
Application Notes
WB: Approx. 55 kDa band observed in human placenta and lung lysates (predicted MW of 56 kDa band according to NP_003949).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNF8

The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Results suggest a novel function of RNF8 as a regulator of RXR alpha-mediated transcriptional activity through interaction between their respective N-terminal regions.

Long Name

Ring Finger Protein 8

Alternate Names

ActA Binding Protein 2

Entrez Gene IDs

9025 (Human)

Gene Symbol

RNF8

UniProt

O76064 (Human)

Additional RNF8 Products

Product Documents for RNF8 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for RNF8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

E3 Ubiquitin Ligases
Epigenetics

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Protocols

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FAQs

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