Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1F3
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Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 2126-2347 of human ROS1(NP_002935) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Novus Biologicals Mouse ROS Antibody (OTI1F3) (NBP2-45626) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ROS Antibody (OTI1F3)
![Western Blot: ROS Antibody (OTI1F3) [NBP2-45626]](https://beta-resources.rndsystems.com/images/products/ROS-Antibody-OTI1F3-Western-Blot-NBP2-45626-img0004.jpg "Western Blot: ROS Antibody (OTI1F3) [NBP2-45626]")
Western Blot: ROS Antibody (OTI1F3) [NBP2-45626]
Western Blot: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS.![Immunohistochemistry-Paraffin: ROS Antibody (OTI1F3) [NBP2-45626]](https://beta-resources.rndsystems.com/images/products/ROS-Antibody-OTI1F3-Immunohistochemistry-Paraffin-NBP2-45626-img0005.jpg "Immunohistochemistry-Paraffin: ROS Antibody (OTI1F3) [NBP2-45626]")
Immunohistochemistry-Paraffin: ROS Antibody (OTI1F3) [NBP2-45626]
Immunohistochemistry-Paraffin: ROS Antibody (OTI1F3) [NBP2-45626] - Staining of HCC78 cells using anti-ROS1 mouse monoclonal antibody (Left). The right is HELA cells as negative control (1:2000).![Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626]](https://beta-resources.rndsystems.com/images/products/ROS-Antibody-OTI1F3-Immunohistochemistry-NBP2-45626-img0001.jpg "Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626]")
Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626]
Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of Carcinoma Human lung tissue.![Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626]](https://beta-resources.rndsystems.com/images/products/ROS-Antibody-OTI1F3-Immunohistochemistry-NBP2-45626-img0002.jpg "Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626]")
Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626]
Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of Carcinoma Human lung tissue.![Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626]](https://beta-resources.rndsystems.com/images/products/ROS-Antibody-OTI1F3-Immunohistochemistry-NBP2-45626-img0003.jpg "Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626]")
Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626]
Immunohistochemistry: ROS Antibody (OTI1F3) [NBP2-45626] - Analysis of Human carcinoma lung tissue.
Western Blot: ROS Antibody (OTI1F3) [NBP2-45626] -
Western Blot: ROS Antibody (OTI1F3) [NBP2-45626] -Analysis of extracts (35ug) from 2 different cell lines by using anti-ROS1 monoclonal antibody (1:500).
Immunohistochemistry-Paraffin: ROS Antibody (OTI1F3) [NBP2-45626] -
Immunohistochemistry-Paraffin: ROS Antibody (OTI1F3) [NBP2-45626] -HCC78 (left) and HeLa (right) xenograft using NBP2-45626 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min)Applications for ROS Antibody (OTI1F3)
Application
Recommended Usage
Immunohistochemistry
1:5000
Immunohistochemistry-Paraffin
1:5000
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ROS1
Long Name
ROS Proto-Oncogene 1, Receptor Tyrosine Kinase
Alternate Names
c-Ros-1, MCF3
Gene Symbol
ROS1
Additional ROS1 Products
Product Documents for ROS Antibody (OTI1F3)
Product Specific Notices for ROS Antibody (OTI1F3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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