RPL7 Antibody

Novus Biologicals | Catalog # NB100-2269

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Orangutan (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RPL7 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 200 and the C-terminus (residue 248) of human Ribosomal Protein L7 using the numbering given in entry NP_000962.2 (GeneID 6129).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPL7 Antibody

Western Blot: RPL7 Antibody [NB100-2269]

Western Blot: RPL7 Antibody [NB100-2269]

Western Blot: RPL7 Antibody [NB100-2269] - Detection of human and mouse RPL7 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TMCK-1, and mouse NIH 3T3 cells prepared using RIPA lysis buffer. Antibody: Affinity purified rabbit anti-RPL7 antibody NB100-2269 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunohistochemistry-Paraffin: RPL7 Antibody [NB100-2269]

Immunohistochemistry-Paraffin: RPL7 Antibody [NB100-2269]

Immunohistochemistry-Paraffin: RPL7 Antibody [NB100-2269] - Mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-RPL7 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: RPL7 Antibody [NB100-2269]

Immunohistochemistry-Paraffin: RPL7 Antibody [NB100-2269]

Immunohistochemistry-Paraffin: RPL7 Antibody [NB100-2269] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-RPL7 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Immunoprecipitation: RPL7 Antibody [NB100-2269]

Immunoprecipitation: RPL7 Antibody [NB100-2269]

Immunoprecipitation: RPL7 Antibody [NB100-2269] - Detection of human RPL7 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-RPL7 antibody NB100-2269(lot NB100-2269-3) used for IP at 6 ug per reaction. RPL7 was also immunoprecipitated by a previous lot of this antibody (lot NB100-2269-2). For blotting immunoprecipitated RPL7, NB100-2269 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for RPL7 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 5 using NB100-2269 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RPL7

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L30P family of ribosomal proteins. It contains an N-terminal basic region-leucine zipper (BZIP)-like domain and the RNP consensus submotif RNP2. In vitro the BZIP-like domain mediates homodimerization and stable binding to DNA and RNA, with a preference for 28S rRNA and mRNA. The protein can inhibit cell-free translation of mRNAs, suggesting that it plays a regulatory role in the translation apparatus. It is located in the cytoplasm. The protein has been shown to be an autoantigen in patients with systemic autoimmune diseases, such as systemic lupus erythematosus. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Alternate Names

humL7-1,60S ribosomal protein L7, MGC117326, ribosomal protein L7

Entrez Gene IDs

6129 (Human); 19989 (Mouse)

Gene Symbol

RPL7

UniProt

P18124 (Human)

Additional RPL7 Products

Product Documents for RPL7 Antibody

Certificate of Analysis

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Product Specific Notices for RPL7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RPL7 Antibody (1)

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  • Name: Sophia Zhang
    Application: Western Blot
    Sample Tested: Muscle
    Species: Mouse
    Verified Customer | Posted 03/28/2014
    WB of RPL7 in muscle
    RPL7 Antibody NB100-2269

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