Rpn2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-30572

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Rpn2 Primary Antibodies »

Product Specifications

Immunogen

between 903 and 953

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rpn2 Antibody - BSA Free

Rpn2 Antibody

Immunohistochemistry;Rpn2 Antibody[NBP3-30572]

Immunohistochemistry;Rpn2 Antibody[NBP3-30572]Detection of mouse PSMD1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- PSMD1 used at a dilution of 1:5,000. Detection: DAB
Rpn2 Antibody

Immunohistochemistry;Rpn2 Antibody[NBP3-30571]

Immunohistochemistry;Rpn2 Antibody[NBP3-30571]Detection of human PSMD1 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PSMD1 used at a dilution of 1:5,000. Detection: DAB
Rpn2 Antibody

Immunoprecipitation:Rpn2 AntibodyNBP3-30572] -

Immunoprecipitation:Rpn2 AntibodyNBP3-30572] -Detection of human PSMD1 by western blot of immunoprecipitates. Samples: Whole cell lysate (200 µg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PSMD1 antibody (NBP3-30572 lot 2) used for IP at 6 µg per reaction. PSMD1 was also immunoprecipitated by a previous lot of this antibody (NBP3-30572 lot 1) and a second.inst a different epitope of PSMD1. For blotting immunoprecipitated PSMD1, NBP3-30572 was used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Applications for Rpn2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Tris-citrate/phosphate buffer, pH 7 to 8

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rpn2

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelmas's syndrome, and Liddle syndrome.

Alternate Names

26S proteasome non-ATPase regulatory subunit 1, 26S proteasome regulatory subunit RPN2, 26S proteasome regulatory subunit S1, 26S proteasome subunit p112, MGC133040, MGC133041, P112, proteasome (prosome, macropain) 26S subunit, non-ATPase, 1, Rpn2, S1

Gene Symbol

PSMD1

Additional Rpn2 Products

Product Documents for Rpn2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Rpn2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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