RPS14 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12555

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RPS14 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human RPS14 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for RPS14 Antibody - BSA Free

Western Blot: RPS14 Antibody [NBP3-12555]

Western Blot: RPS14 Antibody [NBP3-12555]

Western Blot: RPS14 Antibody [NBP3-12555] - Anti-RPS14 rabbit polyclonal antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: RPS14 Antibody [NBP3-12555]

Immunohistochemistry-Paraffin: RPS14 Antibody [NBP3-12555]

Immunohistochemistry-Paraffin: RPS14 Antibody [NBP3-12555] - Staining of human RPS14 in human brain with rabbit polyclonal antibody at 1:200 dilution.
Immunoprecipitation: RPS14 Antibody [NBP3-12555]

Immunoprecipitation: RPS14 Antibody [NBP3-12555]

Immunoprecipitation: RPS14 Antibody [NBP3-12555] - Lane A:0.5 mg 293T Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell LysateLane C:0.5 mg Hela Whole Cell LysateLane D:0.5 mg Jurkat Whole Cell Lysate4 uL anti- RPS14 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti- RPS14 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 17kDaObserved band size :17 kDa
RPS14 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RPS14 Antibody - BSA Free [NBP3-12555] -

Immunocytochemistry/ Immunofluorescence: RPS14 Antibody - BSA Free [NBP3-12555] - Immunofluorescence staining of RPS14 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12555 (dilution ratio 1:200) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Applications for RPS14 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1-5uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPS14

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

Alternate Names

emetine resistance, EMTB40S ribosomal protein S14, ribosomal protein S14

Gene Symbol

RPS14

Additional RPS14 Products

Product Documents for RPS14 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RPS14 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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