RTF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83019

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RTF1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GNHNSKPVYRVAEITGVVETAKVYQLGGTRTNKGLQLRHGNDQRVFRLEFVSNQEFTESEFMKWKEAMFSAGMQLPTLDEINKKELSIKEALNYKFNDQDIEEIVKEKERFRKAPPNYAMKKTQL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RTF1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RTF1 Antibody [NBP1-83019]

Immunocytochemistry/ Immunofluorescence: RTF1 Antibody [NBP1-83019]

Immunocytochemistry/Immunofluorescence: RTF1 Antibody [NBP1-83019] - Immunofluorescent staining of human cell line A-431 shows localization to nucleus & cytosol.
Immunohistochemistry-Paraffin: RTF1 Antibody [NBP1-83019]

Immunohistochemistry-Paraffin: RTF1 Antibody [NBP1-83019]

Immunohistochemistry-Paraffin: RTF1 Antibody [NBP1-83019] - Staining of human skin shows strong nuclear positivity in epidermal cells.

Applications for RTF1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RTF1

In yeast, Rtf1 is a component of the Paf1/RNA polymerase II complex. The Paf1 complex interacts with RNA polymerase II and functions to modify chromatin during transcription. Rtf1 appears to be important for histone H2B ubiquitination by Rad6 and for methylation of histone H3 catalyzed by the Set1/COMPASS complex that functions during transcription elongation.

Alternate Names

KIAA0252GTL7, ortholog of mouse gene trap locus 7, RNA polymerase-associated protein RTF1 homolog, Rtf1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)

Gene Symbol

RTF1

Additional RTF1 Products

Product Documents for RTF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RTF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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