RXR alpha/NR2B1 Antibody (JG99-38)

Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653]

Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of Retinoid X Receptor alpha on MCF-7 cell using anti-Retinoid X Receptor alpha antibody at 1/5,000 dilution.

Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653]

Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Staining Retinoid X Receptor alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653]

Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of paraffin-embedded human esophagus tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.

Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653]

Western blot analysis on different lysates with NBP2-75653 at 1/1,000 dilution. Lane 1: Jurkat cell lysate (low expression) Lane 2: A549 cell lysate Lane 3: HepG2 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 51 kDa Observed band size: 55 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-75653 at 1/1,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653]

Western blot analysis on different lysates with NBP2-75653 at 1/500 dilution. Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Mouse heart tissue lysate Lane 4: Rat liver tissue lysate Lane 5: Rat heart tissue lysate Lysates/proteins at 40 µg/Lane. Predicted band size: 51 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-75653 at 1/500 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.