S Opsin Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-20194

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
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Product Specifications

Immunogen

A synthetic peptide from human OPSB conjugated to blue carrier protein was used as the antigen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for S Opsin Antibody - Azide and BSA Free

Western Blot: S Opsin AntibodyAzide and BSA Free [NBP1-20194]

Western Blot: S Opsin AntibodyAzide and BSA Free [NBP1-20194]

Western Blot: S Opsin Antibody [NBP1-20194] - WB on tissue lysates. Blocking with 1% LFDM for 30 min at RT; Primary antibody used at 1:500 dilution, incubated overnight at 4C.
Immunohistochemistry-Paraffin: S Opsin Antibody - Azide and BSA Free [NBP1-20194]

Immunohistochemistry-Paraffin: S Opsin Antibody - Azide and BSA Free [NBP1-20194]

Immunohistochemistry-Paraffin: S Opsin Antibody [NBP1-20194] - Sections of mouse eye. Tris-EDTA, pH 9 for 20 min using Thermo PT Module. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: S Opsin Antibody - Azide and BSA Free [NBP1-20194]

Immunohistochemistry-Paraffin: S Opsin Antibody - Azide and BSA Free [NBP1-20194]

Immunohistochemistry-Paraffin: S Opsin Antibody [NBP1-20194] - Sections of mouse eye. HIER: Tris-EDTA, pH 9 for 20 min using Thermo PT Module.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. dilution 1: 500, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin.

Applications for S Opsin Antibody - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

10-50 ug/ml

Western Blot

10-50 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID:32716032).

Reviewed Applications

Read 1 review rated 5 using NBP1-20194 in the following applications:

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Reconstitution

Reconstitute 0.5 mg size in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the 0.1 mg size is provided in reconstituted format.

Formulation

Lyophilized from PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S Opsin

FUNCTION: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light.; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.; TISSUE SPECIFICITY: Expresssed in cone photoreceptor cells.

Alternate Names

BCPBlue cone photoreceptor pigmentBlue-sensitive opsin, BOPshort-wave-sensitive opsin 1, CBT, opsin 1 (cone pigments), short-wave-sensitive, s-opsin

Entrez Gene IDs

611 (Human); 12057 (Mouse); 81644 (Rat)

Gene Symbol

OPN1SW

UniProt

P03999 (Human)

Additional S Opsin Products

Product Documents for S Opsin Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for S Opsin Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for S Opsin Antibody - Azide and BSA Free

Customer Reviews for S Opsin Antibody - Azide and BSA Free (1)

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  • S Opsin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse retina
    Species: Mouse
    Verified Customer | Posted 09/29/2017
    s-opsin in mouse retinal lysates. Molecular marker is from Thermo Scientific (Cat # is 26616).
    dilution ratio is 1:200
    S Opsin Antibody - Azide and BSA Free NBP1-20194

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Protocols

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FAQs for S Opsin Antibody - Azide and BSA Free

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  • Q: What is the immunogen sequence of NBP1-20194?

    A: For NBP1-20194, the lab informed me that the immunogen is located in the amino acid region of 200-300 of human Opsin B, it is homologous in many species including guinea pig.

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