S100P Antibody (MSVA-480R)

Novus Biologicals | Catalog # NBP3-57219

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # MSVA-480R
View all S100P Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment corresponding to C-terminal of human S100P protein Swissprot P25815

Localization

Nucleus and cytoplasm

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.

Positive Control
Postive control:Urinary bladder: A strong S100P immunostaining should be seen in the urothelium but not in the stroma or muscular cells..

Negative Control
Negitve control:Kidney: S100P immunostaining should be completely absent.

Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.

Scientific Data Images for S100P Antibody (MSVA-480R)

S100P Antibody (MSVA-480R)

Immunohistochemistry-Paraffin:S100P Antibody (MSVA-480R)NBP3-57219

Muscle invasive urothelial carcinoma of the urinary bladder showing a strong S100P immunostaining of tumor cells.
S100P Antibody (MSVA-480R)

Immunohistochemistry-Paraffin:S100P Antibody (MSVA-480R)NBP3-57219

Kidney cortex S100P immunostaining is completely lacking in the kidney.
S100P Antibody (MSVA-480R)

Immunohistochemistry-Paraffin:S100P Antibody (MSVA-480R)NBP3-57219

Urinary bladder urothelium A strong S100P immunostaining is seen in the urothelium.

Applications for S100P Antibody (MSVA-480R)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Preservative

<0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: S100P

The protein encoded by the S100P gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 4p16. This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate cancer. (provided by RefSeq)

Long Name

S100 Calcium Binding Protein P

Alternate Names

MIG9, migration-inducing gene 9, Protein S100-E, S100 calcium binding protein P, S100 calcium-binding protein Pprotein S100-P, S100E

Gene Symbol

S1P, S100P

Additional S100P Products

Product Documents for S100P Antibody (MSVA-480R)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for S100P Antibody (MSVA-480R)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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