SAM68 Antibody (1O7D1)

Novus Biologicals | Catalog # NBP3-16289

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1O7D1 expressed in HEK293
View all SAM68 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SAM68 (Q07666). MQRRDDPAARMSRSSGRSGSMDPSGAHPSVRQTPSRQPPLPHRSRGGGGGSRGGARASPATQPPPLLPPSATGPDATVGGPAPTPLLPPSATASVKMEPE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SAM68 Antibody (1O7D1) (NBP3-16289) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SAM68 Antibody (1O7D1)

Western Blot: SAM68 Antibody (1O7D1) [NBP3-16289]

Western Blot: SAM68 Antibody (1O7D1) [NBP3-16289]

Western Blot: SAM68 Antibody (1O7D1) [NBP3-16289] - Western blot analysis of extracts of various cell lines, using KHDRBS1/SAM68 Rabbit mAb (NBP3-16289) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry-Paraffin: SAM68 Antibody (1O7D1) [NBP3-16289]

Immunohistochemistry-Paraffin: SAM68 Antibody (1O7D1) [NBP3-16289]

Immunohistochemistry-Paraffin: SAM68 Antibody (1O7D1) [NBP3-16289] - Immunohistochemistry of paraffin-embedded mouse brain using KHDRBS1/SAM68 Rabbit mAb (NBP3-16289) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: SAM68 Antibody (1O7D1) [NBP3-16289]

Immunohistochemistry-Paraffin: SAM68 Antibody (1O7D1) [NBP3-16289]

Immunohistochemistry-Paraffin: SAM68 Antibody (1O7D1) [NBP3-16289] - Immunohistochemistry of paraffin-embedded rat brain using KHDRBS1/SAM68 Rabbit mAb (NBP3-16289) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: SAM68 Antibody (1O7D1) [NBP3-16289]

Immunohistochemistry-Paraffin: SAM68 Antibody (1O7D1) [NBP3-16289]

Immunohistochemistry-Paraffin: SAM68 Antibody (1O7D1) [NBP3-16289] - Immunohistochemistry of paraffin-embedded human colon using KHDRBS1/SAM68 Rabbit mAb (NBP3-16289) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
SAM68 Antibody (1O7D1)

Immunocytochemistry/ Immunofluorescence: SAM68 Antibody (1O7D1) [NBP3-16289] -

Immunocytochemistry/ Immunofluorescence: SAM68 Antibody (1O7D1) [NBP3-16289] - Confocal imaging of C6 cells using SAM68 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Applications for SAM68 Antibody (1O7D1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SAM68

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export Function: Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase

Long Name

Src-associated in Mitosis 68 kDa Protein

Alternate Names

KHDRBS1, p62, p68

Gene Symbol

KHDRBS1

Additional SAM68 Products

Product Documents for SAM68 Antibody (1O7D1)

Certificate of Analysis

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Product Specific Notices for SAM68 Antibody (1O7D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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