SAP102 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87691

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (98%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SAP102 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: HKHQHCCKCPECYEVTRLAALRRLEPPGYGDWQVPDPYGPGGGNGASAGYGGYSSQTLPSQAGATPTPRTKAKLIPTGRDVGPVPPKPVPGKSTPKLNGSGPSWWPECTCTNRDWYEQVNGSD

Reactivity Notes

Reactivity reported in scientific literature (PMID: 19167192)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SAP102 Antibody - BSA Free

Western Blot: SAP102 Antibody [NBP1-87691]

Western Blot: SAP102 Antibody [NBP1-87691]

Western Blot: SAP102 Antibody [NBP1-87691] - Analysis in control (vector only transfected HEK293T lysate) and DLG3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
SAP102 Antibody - BSA Free Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691]

Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691]

Staining of human tonsil shows no positivity in glandular cells as expected.
SAP102 Antibody - BSA Free Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691]

Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691]

Staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.
SAP102 Antibody - BSA Free Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691]

Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691]

Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
SAP102 Antibody - BSA Free Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691]

Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691]

Staining of human colon shows no positivity in glandular cells as expected.
SAP102 Antibody - BSA Free Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691]

Immunohistochemistry-Paraffin: SAP102 Antibody [NBP1-87691]

Staining of human hippocampus shows strong cytoplasmic positivity in neurons.

Applications for SAP102 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAP102

Synapse-Associated Protein 102 (SAP102) is one of a family of plasma membrane-associated proteins found in synaptic junctions. Like other members of the family, SAP102 has three ~90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticity. SAP102 is believed to participate in the clustering of certain proteins, including NMDA receptors, shaker-type potassium channels at the synaptic membrane in CNS neurons. NMDA receptors and Shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits) may interact with SAP102 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with SAP102 in vitro through a pseudo-homotypic PDZ-PDZ interaction.

Alternate Names

discs, large homolog 3 (Drosophila), KIAA1232discs, large homolog 3 (neuroendocrine-dlg, Drosophila), MRX90MRX, NEDLGdisks large homolog 3, Neuroendocrine-DLG, SAP-102, SAP102NE-Dlg, Synapse-associated protein 102, XLMR

Gene Symbol

DLG3

Additional SAP102 Products

Product Documents for SAP102 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SAP102 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SAP102 Antibody - BSA Free

Customer Reviews for SAP102 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review SAP102 Antibody - BSA Free and earn rewards!

Have you used SAP102 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies