SAR1B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32725

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SAR1B Primary Antibodies »

Product Specifications

Immunogen

Full length human SAR1B Recombinant protein.

Localization

Endoplasmic reticulum membrane, Peripheral membrane protein, Golgi apparatus, Golgi stack membrane, Peripheral membrane protein

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SAR1B Antibody - BSA Free

Western Blot: SAR1B Antibody [NBP1-32725]

Western Blot: SAR1B Antibody [NBP1-32725]

Western Blot: SAR1B Antibody [NBP1-32725] - Adult mouse small intestine homogenate, 20 ug total protein per lane. Antibody at 1:1000. Secondary HRP conjugated antibody at 1:5000. Bands visualized with Immobilon Western Chemiluminescence HRP substrate, imaged with Syngene system. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: SAR1B Antibody [NBP1-32725]

Immunocytochemistry/ Immunofluorescence: SAR1B Antibody [NBP1-32725]

Immunocytochemistry/Immunofluorescence: SAR1B Antibody [NBP1-32725] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725]

Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725]

Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725] - Mouse ovary. SAR1B antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: SAR1B Antibody [NBP1-32725]

Western Blot: SAR1B Antibody [NBP1-32725]

Western Blot: SAR1B Antibody [NBP1-32725] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE, antibody diluted at 1:3000.
Western Blot: SAR1B Antibody [NBP1-32725]

Western Blot: SAR1B Antibody [NBP1-32725]

Western Blot: SAR1B Antibody [NBP1-32725] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE, antibody diluted at 1:1000.
Western Blot: SAR1B Antibody [NBP1-32725]

Western Blot: SAR1B Antibody [NBP1-32725]

Western Blot: SAR1B Antibody [NBP1-32725] - A. 50 ug rat brain lysate/extract 12 % SDS-PAGE SAR1B antibody dilution: 1:3000
Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725]

Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725]

Immunohistochemistry-Paraffin: SAR1B Antibody [NBP1-32725] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.

Applications for SAR1B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
SAR1B antibody validated for WB from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP1-32725 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SAR1B

SAR1B belongs to the small GTPase superfamily, SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus and is activated by the guanine nucleotide exchange factor PREB. SAR1B is involved in the selection of the protein cargo and the assembly of the COPII coat complex. Defects in SAR1B are the cause of chylomicron retention disease (CMRD). CMRD is an autosomal recessive disorder of severe fat malabsorption associated with failure to thrive in infancy. The conditions are characterized by deficiency of fat-soluble vitamins, low blood cholesterol levels, and a selective absence of chylomicrons from blood. Affected individuals accumulate chylomicron-like particles in membrane-bound compartments of enterocytes, which contain large cytosolic lipid droplets.

Alternate Names

2310075M17Rik, ANDD, CMRD, EC 3.6.5, GTP-binding protein B, GTP-binding protein SAR1b, GTP-binding protein Sara, SAR1 homolog B (S. cerevisiae), SAR1a gene homolog (S. cerevisiae) 2, SAR1a gene homolog 2, SAR1a gene homolog 2 (S. cerevisiae), SARA2GTBPB, SARB

Entrez Gene IDs

51128 (Human); 66397 (Mouse); 287276 (Rat)

Gene Symbol

SAR1B

UniProt

Q9Y6B6 (Human)

Additional SAR1B Products

Product Documents for SAR1B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SAR1B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for SAR1B Antibody - BSA Free

Customer Reviews for SAR1B Antibody - BSA Free (1)

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Customer Images

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  • SAR1B Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Adult small intestine
    Species: Mouse
    Verified Customer | Posted 10/23/2019
    Mouse small intestine was homogenised and protein content was quantified by a BCA assay. Twenty micrograms of protein were resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membranes. Membranes were probed with primary antibody Sar1B diluted 1:1000 in 5% BSA 2, before incubation with anti rabbit secondary horseradish peroxidase-conjugated antibody 1:5000. Blots were visualised with Immobilon Western Chemiluminescence HRP Substrate and imaged with Syngene chemiluminescence imaging system.
    SAR1B Antibody - BSA Free NBP1-32725

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Protocols

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