SASH1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85183

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SASH1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLSAKSSTEPSLKSFSRNQLGNYPTLPLMKSGDALKQGQEEGRLGGGLAPDTSKSCDPPGVTGLNKNRRSLPVSICRSCETLEGPQTVDTWPRSH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SASH1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SASH1 Antibody [NBP1-85183]

Immunocytochemistry/ Immunofluorescence: SASH1 Antibody [NBP1-85183]

Immunocytochemistry/Immunofluorescence: SASH1 Antibody [NBP1-85183] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: SASH1 Antibody [NBP1-85183]

Immunohistochemistry-Paraffin: SASH1 Antibody [NBP1-85183]

Immunohistochemistry-Paraffin: SASH1 Antibody [NBP1-85183] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Applications for SASH1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SASH1

SASH1 (SAM and SH3 domain containing 1) was identified in an in silico analysis of loss of heterozygosity (LOH) to identify genes downregulated in breast cancer. Since this discovery, it has also been found to be a candidate tumor suppressor in colon cancer. Structurally, SASH1 contains two SAM (sterile alpha motif) domains and one SH3 domain indicating a role in signal transduction and as a signaling adapter.

Alternate Names

dJ323M4, dJ323M4.1, KIAA0790RP3-323M4.1, PEPE1, Proline-glutamate repeat-containing protein, SAM and SH3 domain containing 1,2500002E12Rik, SAM and SH3 domain-containing protein 1, SH3D6A

Gene Symbol

SASH1

Additional SASH1 Products

Product Documents for SASH1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SASH1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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