SC35 Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-47290
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Scientific Data Images for SC35 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: SC35 Antibody [NBP2-47290]
Immunocytochemistry/Immunofluorescence: SC35 Antibody [NBP2-47290] - Staining of human cell line U-251 MG shows localization to nuclear speckles & cytosol. Antibody staining is shown in green.Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290]
Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290]
Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290]
Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290]
Immunohistochemistry-Paraffin: SC35 Antibody [NBP2-47290] - Staining of human tonsil shows moderate to strong positivity in germinal center cells.Applications for SC35 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Paraffin
Reviewed Applications
Read 1 review rated 1 using NBP2-47290 in the following applications:
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Background: SC35
Alternate Names
Gene Symbol
Additional SC35 Products
Product Documents for SC35 Antibody - BSA Free
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Product Specific Notices for SC35 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SC35 Antibody - BSA Free
Customer Reviews for SC35 Antibody - BSA Free (1)
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Application: Immunohistochemistry-FrozenSample Tested: LiverSpecies: MouseVerified Customer | Posted 12/20/2016This customer tested the product in an untested application (IHC-Fr) and earned the Innovator's Reward for this product. Antibody Storage Conditions: -20℃ Staining Expectations: positive signals should be within nucleus Fixative Composition: PFA Fixation Time & Temperature: 15 min RT Tissue Processing: as normal Blocking Solution: 5% goat serum in PBS Time & Temperature: 15min RT Primary Antibody: Dilution: 1:50 Diluent Buffer: 5% goat serum in PBS Time & Temperature: 15min RT Washing Conditions: Wash Buffer Composition: PBS Times/Washing: 5min x 3 times Repetitions: 3 Secondary Antibody Manufacturer and Catalog #: life technologies Ref:A11034 Secondary description: goat anti Rabit Dilution: 1:2000 Diluent Buffer: 5% goat serum in PBS Incubation Time & Temperature: 1 hour RT Post-Secondary Washing: PBS Detection Method: Detection: confocal Procedure: as olypus DAB-Incubation Time (if applicable): Click here to enter text. Controls: Positive Control: No Negative Control: no
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars