Key Product Details

Species Reactivity

Human, Mouse, Rat, Primate

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

FL490

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # K74/71
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Product Specifications

Immunogen

Fusion protein amino acids 1929-2009 (cytoplasmic C-terminus) of rat Scn1a (Uniprot#: P04774) produced recombinantly in E. Coli

Specificity

No cross-reactivity with Nav1.2, Nav1.3 and Nav1.9

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

FL490 Excitation: 491 nm, Emission: 515nm.
Each order is a unique lot. For specialized requirements, please inquire for available options.

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone K74/71 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone K74/71 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media and conjugated.

Applications for Scn1a Antibody (K74/71) [FL490] - Chimeric

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

1X PBS (pH 7.4)

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Scn1a

Voltage-dependent sodium channels are heteromeric complexes that regulate sodium exchange between intracellular and extracellular spaces and are essential for the generation and propagation of action potentials in muscle cells and neurons. Each sodium channel is composed of a large pore-forming, glycosylated alpha subunit and two smaller beta subunits. This gene encodes a sodium channel alpha subunit, which has four homologous domains, each of which contains six transmembrane regions. Allelic variants of this gene are associated with generalized epilepsy with febrile seizures and epileptic encephalopathy. Alternative splicing results in multiple transcript variants. The RefSeq Project has decided to create four representative RefSeq records. Three of the transcript variants are supported by experimental evidence and the fourth contains alternate 5' untranslated exons, the exact combination of which have not been experimentally confirmed for the full-length transcript. [provided by RefSeq, Oct 2015]

Alternate Names

brain I alpha subunit, DEE6, DEE6A, DEE6B, DRVT, EIEE6, FEB3, FEB3A, FHM3, GEFSP2, HBSCI, NAC1, Nav1.1, SCN1, SMEI, sodium channel, voltage-gated, type I, alpha polypeptide, sodium channel, voltage-gated, type I, alpha subunit

Gene Symbol

SCN1A

Additional Scn1a Products

Product Documents for Scn1a Antibody (K74/71) [FL490] - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Scn1a Antibody (K74/71) [FL490] - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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